ANTIBODIES

Contributor Information
- Name Karen Pulford
- Institute University of Oxford
Tool Details
- Tool name: Anti-Neutrophil elastase [NP57]
- Clone: NP57
- Tool type: Antibodies
- Tool sub-type: Primary antibody
- Class: Monoclonal
- Conjugate: Unconjugated
- Reactivity: Human
- Host: Mouse
- Molecular weight of the target: 150 kDa (full length)
- Application: IHC ; IF ; IP ; WB
- Description: Monoclonal antibody with use in diagnosis of acute leukaemia and tumour deposits in myeloid leukaemia.
- Immunogen: Neutrophil granule proteins
- Immunogen UniProt ID: P08246
- Isotype: IgG1 kappa
- Research area: Cancer; Cell signaling and signal transduction; Immunology
- Myeloma used: P3/NS1/1-Ag4.1
- For Research Use Only
Target Details
- Target: Neutrophil elastase
- Target molecular weight: 150 kDa (full length)
- Target background: Human Neutrophil Elastase (HNE) belongs to the chymotrypsin family of serine proteases able to solubilize fibrous elastin, an important extracellular matrix protein that has the unique property of elastic recoil, and plays a major structural function in lungs, arteries, skin and ligaments. HNE is primarily located in the azurophil granules of polymorphonuclear leukocytes, but has also been detected in nuclear membrane, Golgi complex, ER and the mitochondria of these cells. The enzyme is involved in the tissue destruction and inflammation and implicated in numerous diseases, including emphysema, chronic obstructive pulmonary disease, cystic fibrosis, ischemic-reperfusion injury and rheumatoid arthritis. NP57 is useful for the differential diagnosis of acute leukaemia by APAAP labelling of cell smears. It may also be of value for the histopathological diagnosis of tumour deposits in myeloid leukaemia and for the detection of neutrophils in paraffin sections.
Application Details
- Application: IHC ; IF ; IP ; WB
Handling
- Format: Liquid
- Concentration: 1 mg/ml
- Storage buffer: PBS with 0.02% azide
- Storage conditions: Store at -20ðC frozen. Avoid repeated freeze / thaw cycles
- Shipping conditions: Shipping at 4ðC
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References
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