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Contributor Information

  • Name Jacqueline Cordell
  • Institute University of Oxford

Tool Details

  • Tool name: Anti-CD68 [Y1/82A] mAb
  • Alternate names: CD68 Molecule; CD68 Antigen; Macrophage Antigen CD68; GP11; Scavenger Receptor Class D; Member; Scavenger Receptor Class D; SCARD1; LAMP4
  • Clone: Y1/82A
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Human
  • Host: Mouse
  • Application: FACS ; IHC ; IF ; WB
  • Description: Monoclonal antibody directed against CD68, which can aid diagnosis of monocytic leukaemia's and histiocytic neoplasms and help identify macrophages in tissues from various inflammatory and neoplastic disease states.
  • Immunogen: Lysosomal contents of lung macrophages
  • Immunogen UniProt ID: P34810
  • Isotype: IgG2a
  • Research area: Cell biology; Immunology; Stem cell biology
  • Myeloma used: P3/NS1/1-Ag4.1

  • For Research Use Only

Target Details

  • Target: CD68
  • Target background: CD68 is a 110 kD glycoprotein, also known as macrosialin, belonging to the sialomucin family. It is closely related to the family of acidic, highly glycosylated lysosomal-associated membrane proteins (LAMPs). CD68 is predominately expressed in cytoplasmic granules of monocytes/macrophages, dendritic cells, and granulocytes. It is one of the useful myeloid cell markers. Further studies have shown that CD68 is also expressed by a subset of hematopoietic progenitors, ?/d T cells, NK cells, LAK cells, subset of B cells, fibroblasts, and endothelial cells. The biological function of CD68 is still unknown. Anti-CD68 (Y1/82A) reacted with peripheral blood and bone marrow monocytes and resident macrophages in all tissues tested. Anti-CD68 did not react with other cell types, apart from some osteoclasts and megakaryocytes. This antibody was shown to be useful in characterising monocytic leukaemia's, histiocytic neoplasms and detecting macrophages in disease.

Application Details

  • Application: FACS ; IHC ; IF ; WB

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: Store at -20° C frozen. Avoid repeated freeze / thaw cycles
  • Shipping conditions: Shipping at 4° C

Documentation

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References

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  • •  Lee et al. 2018. Sci Rep. 8(1):15076. PMID: 30305672.
  • •  Matossian et al. 2018. Breast Cancer Res Treat. 169(2):381-390. PMID: 29392581.
  • •  Ferguson et al. 2018. Front Immunol. 9:1802. PMID: 30127787.
  • •  Ferguson et al. 2018. Front Immunol. 9:1802. PMID: 30127787.
  • •  MacParland et al. 2017. ACS Nano. :. PMID: 28040885.
  • •  Phenotype Determines Nanoparticle Uptake by Human Macrophages from Liver and Blood.
  • •  Conrad et al. 2015. Arthritis Res Ther. 17:179. PMID: 26178906.
  • •  In vivo pre-activation of monocytes in patients with axial spondyloarthritis.
  • •  Caete et al. 2015. Arthritis Res Ther. 17:173. PMID: 26156866.
  • •  Ectopic lymphoid neogenesis is strongly associated with activation of the IL-23 pathway in rheumatoid synovitis.
  • •  Doussis et al. 1993. J Clin Pathol. 46(4):334-6. PMID: 7684403.
  • •  CD68 reactivity of non-macrophage derived tumours in cytological specimens.
  • •  Pulford et al. 1990. Int Immunol. 2(10):973-80. PMID: 2078523.
  • •  Distribution of the CD68 macrophage/myeloid associated antigen.
  • •  Micklem et al. 1989. Br J Haematol. 73(1):6-11. PMID: 2803980.
  • •  A human macrophage-associated antigen (CD68) detected by six different monoclonal antibodies.
  • •  Pulford et al. 1989. J Clin Pathol. 42(4):414-21. PMID: 2654191.
  • •  KP1: a new monoclonal antibody that detects a monocyte/macrophage associated antigen in routinely processed tissue sections.