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Contributor Information

  • Name Karen Pulford
  • Institute University of Oxford

Tool Details

  • Tool name: Anti-CD68 [KP1]
  • Alternate names: CD68 Molecule; CD68 Antigen; Macrophage Antigen CD68; GP11; Scavenger Receptor Class D; Member; Scavenger Receptor Class D; SCARD1; LAMP4
  • Clone: KP1
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Human
  • Host: Mouse
  • Application: FACS ; IHC ; IF ; IP ; WB
  • Description: Monoclonal antibody which recognises most human macrophages, useful in studying macrophage disorders including both reactive and neoplastic states.
  • Immunogen: Lysosomal contents of lung macrophages
  • Immunogen UniProt ID: P34810
  • Isotype: IgG1
  • Research area: Cell biology; Immunology; Stem cell biology
  • Myeloma used: P3/NS1/1-Ag4.1

  • For Research Use Only

Target Details

  • Target: CD68
  • Target background: Anti-CD68 (KP1) monoclonal antibody recognises a fixation-resistant epitope in a wide variety of tissue macrophages (such as Kupffer cells germinal centre, splenic, and lamina propria macrophages), and in granulocyte precursors. Anti-CD68 (KP1) recognises most human macrophages in routinely processed tissue, where the target can be identified by immunocytochemical techniques. CD68 is a 110-kD transmembrane glycoprotein which is highly expressed by human monocytes and tissue macrophages. It is a member of the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family and favours binding to lectins through its heavily glycosylated extracellular domain. CD68 is also a member of a family of hematopoietic mucin-like molecules that includes leukosialin/CD43 and stem cell antigen CD34. CD68 is resistant to conventional fixatives such as formalin and B5, as such KP1 may be of practical importance for diagnostic pathologists. CD68 persists throughout cell maturation from the monocyte to the mature macrophage stage. The quantity of this antigen increases upon macrophages when cells are activated and mature indicated by a greater intensity of staining. KP1 is widely used to detect macrophages in paraffin embedded tissue sections.

Application Details

  • Application: FACS ; IHC ; IF ; IP ; WB

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: Store at -20°C frozen. Avoid repeated freeze / thaw cycles
  • Shipping conditions: Shipping at 4°C

Documentation

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References

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