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Contributor Information

  • Name Jesper Svejstrup
  • Institute Cancer Research UK, London Research Institute: Clare Hall Laboratories

Tool Details

  • Tool name: Anti-Phospho RNA polII (Ser5) [CTD 8A7]
  • Clone: CTD 8A7
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Human ; Saccharomyces cerevisiae
  • Host: Mouse
  • Application: IF ; ELISA ; FACS ; IF ; WB
  • Strain: Balb/c
  • Description: RNAPII is an essential component of the RNAP II transcription elongation complex. Recruitment of RNA processing factors to the elongation complex is coordinated by the phosphorylation of Serine-2 and Serine-5 residues within the C-terminal repeat (YSPTSPS) domain of the largest subunit of RNAPII.
  • Immunogen: Peptide: 10 repeats of synthetic peptide YSPTSPS using chemically sythesized phospho-Ser5.
  • Isotype: IgM
  • Research area: Genetics
  • Myeloma used: Sp2/0-Ag14

  • For Research Use Only

Target Details

  • Target: RNA Polymerase II (Phosphorylated)
  • Target background: RNAPII is an essential component of the RNAP II transcription elongation complex. Recruitment of RNA processing factors to the elongation complex is coordinated by the phosphorylation of Serine-2 and Serine-5 residues within the C-terminal repeat (YSPTSPS) domain of the largest subunit of RNAPII.

Application Details

  • Application: IF ; ELISA ; FACS ; IF ; WB

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: -15°C to -25°C
  • Shipping conditions: Shipping at 4°C

Documentation

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References

  • •  Szafran et al. 2017. PLoS One. 12(7):e0180141. PMID: 28704378.
  • •  Trevio et al. 2016. Mol Endocrinol. 30(2):158-72. PMID: 26652902.
  • •  Benferhat et al. 2012. J Virol. 86(20):11333-44. PMID: 22896612.
  • •  Large-scale chromatin immunoprecipitation with promoter sequence microarray analysis of the interaction of the NSs protein of Rift Valley fever virus with regulatory DNA regions of the host genome.
  • •  Alm-Kristiansen et al. 2011. Mol Cancer. 10:21. PMID: 21338522.
  • •  PIAS1 interacts with FLASH and enhances its co-activation of c-Myb.
  • •  Aune et al. 2008. Clin Cancer Res. 14(20):6449-55. PMID: 18927284.
  • •  Von Hippel-Lindau-coupled and transcription-coupled nucleotide excision repair-dependent degradation of RNA polymerase II in response to trabectedin.
  • •  Otero et al. 1999. Mol Cell. 3(1):109-18. PMID: 10024884.
  • •  Elongator, a multisubunit component of a novel RNA polymerase II holoenzyme for transcriptional elongation.