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Contributor Information

  • Name Karen Pulford
  • Institute University of Oxford

Tool Details

  • Tool name: Anti-CEACAM5&6 [By114]
  • Alternate names: Carcinoembryonic Antigen Related Cell Adhesion Molecule 5 & 6; Meconium Antigen 1; CEA; Carcinoembryonic Antigen; CD66e & c
  • Clone: By114
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Human
  • Host: Mouse
  • Application: FACS ; IHC ; IF ; IP ; WB
  • Description: Clone By114 reacts with a phosphatidylinositol (PI) linked protein with a molecular mass of 90 kD on granulocyte and squamous epithelium. This antibody is useful in differentiating normal cells from neoplastic cells of granulocyte origin. CD66CE is useful in differentiating normal cells from neoplastic cells of granulocyte origin. By114 may be used to study Paroxysmal Nocturnal Hemoglobinuria (PNH).
  • Immunogen: B cell Lymphoma cells
  • Isotype: IgG1 kappa
  • Research area: Cancer; Cell biology
  • Myeloma used: P3/NS1/1-Ag4.1

  • For Research Use Only

Target Details

  • Target: Carcinoembryonic antigen-related cell adhesion molecules 5 & 6 (CEACAM5, CD66e; CEACAM6, CD66c)CD66 C and E subtypes (NCA)
  • Target background: Clone By114 reacts with a phosphatidylinositol (PI) linked protein with a molecular mass of 90 kD on granulocyte and squamous epithelium. This antibody is useful in differentiating normal cells from neoplastic cells of granulocyte origin. CD66CE is useful in differentiating normal cells from neoplastic cells of granulocyte origin. By114 may be used to study Paroxysmal Nocturnal Hemoglobinuria (PNH).

Application Details

  • Application: FACS ; IHC ; IF ; IP ; WB

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: -15°C to -25°C
  • Shipping conditions: Shipping at 4°C

Documentation

References

  •   Tooze et al. 1995. Exp Hematol. 23(14):1484-91. PMID: 8542935.
  •   The novel monoclonal antibody By114 helps detect the early emergence of a paroxysmal nocturnal hemoglobinuria clone in aplastic anemia.
  •   Mayne et al. 1993. Br J Haematol. 83(1):30-8. PMID: 8435334.
  •   Antibody By114 is selective for the 90 kD PI-linked component of the CD66 antigen: a new reagent for the study of paroxysmal nocturnal haemoglobinuria.