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Contributor Information

  • Name Gerard Evan
  • Institute University of California San Francisco

Tool Details

  • Tool name: Anti-cMyc [9E10]
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Human
  • Host: Mouse
  • Cancer type: Gynaecologic cancer; Digestive / Gastrointestinal cancer; Breast cancer; Lung
  • Application: ChIP ; ELISA ; IHC ; IP ; WB
  • Strain: Balb/c
  • Description: Monoclonal antibody raised against sequence of c-Myc, a proto-oncogene heavily implicated in a variety of (hematopoietic) cancers, which has been used in cancer research to detect and understand the distribution of the c-Myc oncogene product.
  • Immunogen: Synthetic peptide (AEEQKLISEEDLLRKRREQLKHKLEQLRNSCA - 32 aa) corresponding to 408-439 of Human c-Myc conjugated to KLH.
  • Immunogen UniProt ID: P01106
  • Isotype: IgG1 kappa
  • Research area: Cancer; Cell biology; Genetics; Stem cell biology
  • Myeloma used: Sp2/0-Ag14

  • For Research Use Only

Target Details

  • Target: c Myc epitope tag
  • Target background: Anti c-Myc is raised against the proto-oncogene c-Myc, analogous to the avian v-Myc gene a very strong proto-oncogene often found to be upregulated in cancer. Aberrant and constitutive expression of c-Myc has been particularly implicated in the upregulation of genes involved in cellular proliferation and has been identified in cancers including carcinoma of cervix, colon, breast, lung and stomach, plus a translocation leading to Burkitt's lymphoma. The gene product of c-Myc (p62) is upregulated in various hematopoietic tumours. 9E10 recognises recombinant proteins incorporating a c-Myc epitope tag and detects human c-Myc protein as well as a peptide in random coil configuration not as a helix. It recognises overexpressed proteins containing the amino acid 408-439 sequence, of the human c-Myc gene product. c-Myc is believed to regulate expression of 15% of all genes, hence its strong implicated in cancer and represents a promising therapeutic target. c-Myc has been shown to be involved in apoptosis, cell cycle progression, proliferation and differentiation. c-Myc carries out both its healthy and oncogenic functions within the nucleus. The c-Myc protein is a 62 kDa transcription factor encoded by c-Myc gene on human chromosome 8q24. Anti c-Myc was created to precipitate p62 from human cells and recognise the c-Myc gene product, particularly in immunoblots to enable quantification of c-Myc in cells.

Application Details

  • Application: ChIP ; ELISA ; IHC ; IP ; WB

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: Store at -20°C frozen. Avoid repeated freeze / thaw cycles
  • Shipping conditions: Shipping at 4°C

Documentation

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References

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  • •  Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product.