Product Image

Contributor Information

  • Name Karen Pulford
  • Institute University of Oxford

Tool Details

  • Tool name: Anti-SIGLEC2 [4KB128]
  • Clone: 4KB128
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Human
  • Host: Mouse
  • Application: FACS
  • Description: SIGLEC-2 is first expressed in the cytomplasm of pre- and pro- B cells. SIGLEC-2 is broadly expressed in normal and neoplastic B cells and absent from other leucocytes and tissues. SIGLEC-2 is a member of the immunoglobulin superfamily and serves as an adhesion receptor for sialic acid-bearing ligands expressed on erythrocytes and all leukocyte classes. It also associates with tyrosine kinases and play a role in signal transduction and B-cell activation. SIGLEC-2 is strongly expressed in hairy cell leukaemias.
  • Immunogen: B cell lymphoma cells
  • Isotype: IgG1 kappa
  • Research area: Cancer; Immunology; Stem cell biology
  • Myeloma used: P3/NS1/1-Ag4.1

  • For Research Use Only

Target Details

  • Target: Sialic acid binding Ig-like lectin 2 (SIGLEC2, CD22, BL-CAM)
  • Target background: SIGLEC-2 is first expressed in the cytomplasm of pre- and pro- B cells. SIGLEC-2 is broadly expressed in normal and neoplastic B cells and absent from other leucocytes and tissues. SIGLEC-2 is a member of the immunoglobulin superfamily and serves as an adhesion receptor for sialic acid-bearing ligands expressed on erythrocytes and all leukocyte classes. It also associates with tyrosine kinases and play a role in signal transduction and B-cell activation. SIGLEC-2 is strongly expressed in hairy cell leukaemias.

Application Details

  • Application: FACS

Handling

  • Format: Liquid
  • Concentration: 0.9-1.1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: -15°C to -25°C
  • Shipping conditions: Shipping at 4°C

Documentation

Related Tools

Product Image

Anti-Calcineurin [BF1.1]

#151536
Product Image

Anti-Cytokeratin 18 [DEK18]

#154746
Product Image

Anti-Erythrocytes [OX83]

#156539

References

  • •  Law et al. 1994. Immunol Today. 15(9):442-9. PMID: 7945784.
  • •  Regulation of lymphocyte activation by the cell-surface molecule CD22.
  • •  Mason et al. 1987. Blood. 69(3):836-40. PMID: 3101766.
  • •  Value of monoclonal anti-CD22 (p135) antibodies for the detection of normal and neoplastic B lymphoid cells.
  • •  Campana et al. 1985. J Immunol. 134(3):1524-30. PMID: 3918103.
  • •  Human B cell development. I. Phenotypic differences of B lymphocytes in the bone marrow and peripheral lymphoid tissue.