Product Image

Contributor Information

  • Name Helen Turley
  • Institute University of Oxford

Tool Details

  • Tool name: Anti-PHD3 [EG188e/d5]
  • Clone: EG188e/d5
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Human
  • Host: Mouse
  • Molecular weight of the target: 27.3 kDa
  • Application: IHC ; WB
  • Strain: Balb/c
  • Description: PHD3 catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins, hydroxylates HIF-1 alpha at Pro-564, and also hydroxylates HIF-2 alpha. It functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitylation complex. It may play a role in cell growth regulation in muscle cells and in apoptosis in neuronal tissue. It promotes cell death through a caspase-dependent mechanism
  • Immunogen: Residues 1-100 of human PHD3
  • Isotype: IgG1
  • Research area: Cancer; Tissue-specific biology; Genetics; Metabolism
  • Myeloma used: P3/NS1/1-Ag4.1

  • For Research Use Only

Target Details

  • Target: Prolyl Hydroxylase 3 (PHD3)
  • Target molecular weight: 27.3 kDa
  • Target background: PHD3 catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins, hydroxylates HIF-1 alpha at Pro-564, and also hydroxylates HIF-2 alpha. It functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitylation complex. It may play a role in cell growth regulation in muscle cells and in apoptosis in neuronal tissue. It promotes cell death through a caspase-dependent mechanism

Application Details

  • Application: IHC ; WB

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: -15°C to -25°C
  • Shipping conditions: Shipping at 4°C

Documentation

Related Tools

Product Image

Anti-PAX6 [AD1.5]

#156641
Product Image

Anti-OX74 [OX74]

#156600
Product Image

Anti-SIGLEC1 [HSn 7D2]

#151199

References

  • •  Fujita et al. 2012. J Biol Chem. 287(47):39942-53. PMID: 22948157.
  • •  Prolyl hydroxylase 3 (PHD3) modulates catabolic effects of tumor necrosis factor-a (TNF-a) on cells of the nucleus pulposus through co-activation of nuclear factor B (NF-B)/p65 signaling.
  • •  Soilleux et al. 2005. Histopathology. 47(6):602-10. PMID: 16324198.
  • •  Use of novel monoclonal antibodies to determine the expression and distribution of the hypoxia regulatory factors PHD-1, PHD-2, PHD-3 and FIH in normal and neoplastic human tissues.
  • •  Stolze et al. 2004. J Biol Chem. 279(41):42719-25. PMID: 15302861.
  • •  Appelhoff et al. 2004. J Biol Chem. 279(37):38458-65. PMID: 15247232.
  • •  Genetic analysis of the role of the asparaginyl hydroxylase factor inhibiting hypoxia-inducible factor (FIH) in regulating hypoxia-inducible factor (HIF) transcriptional target genes [corrected].
  • •  Differential function of the prolyl hydroxylases PHD1, PHD2, and PHD3 in the regulation of hypoxia-inducible factor.