Product Image

Contributor Information

  • Name Karen Vousden
  • Institute Cancer Research UK, Glasgow: The Beatson Institute

Tool Details

  • Tool name: Anti-TIGAR [9C10]
  • Clone: 9C10
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Human
  • Host: Mouse
  • Molecular weight of the target: 30 kDa
  • Application: ChIP ; IP ; WB
  • Description: TIGAR (TP53 induced glycolysis and apoptosis regulator) is a novel p53-inducible gene, that shows similarity to the bisphosphatase domain of the bifunctional enzyme 6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase, the main regulator of glycolysis. TIGAR functions to lower fructose-2,6-bisphosphate levels in cells, resulting in an inhibition of glycolysis, and an overall decrease in intracellular reactive oxygen species (ROS) levels, correlating with a decreased sensitivity to apoptosis.
  • Immunogen: A 15-amino acid peptide corresponding to the exon COOH-terminal region of human TIGAR protein (CMNLQDHLNGLTETR).
  • Isotype: IgG2b
  • Research area: Apoptosis and autophagy; Cancer; Genetics
  • Myeloma used: Sp2/0-Ag14

  • For Research Use Only

Target Details

  • Target: TIGAR
  • Target molecular weight: 30 kDa
  • Target background: TIGAR (TP53 induced glycolysis and apoptosis regulator) is a novel p53-inducible gene, that shows similarity to the bisphosphatase domain of the biFn enzyme 6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase, the main regulator of glycolysis. TIGAR functions to lower fructose-2,6-bisphosphate levels in cells, resulting in an inhibition of glycolysis, and an overall decrease in intracellular reactive oxygen species (ROS) levels, correlating with a decreased sensitivity to apoptosis.

Application Details

  • Application: ChIP ; IP ; WB

Handling

  • Format: Liquid
  • Concentration: 0.9-1.1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: -15°C to -25°C
  • Shipping conditions: Shipping at 4°C

Documentation

Related Tools

Product Image

Anti-CD8 [14] rAb

#153256
Product Image

Anti-Striated Muscle Myosin Heavy Chains (SM1 and SM2) [83B6]

#151595
Product Image

Anti-OX87 [OX87]

#156617

References

  • •  Bensaad et al. 2006. Cell. 126(1):107-20. PMID: 16839880.
  • •  TIGAR, a p53-inducible regulator of glycolysis and apoptosis.