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Contributor Information

  • Institute European Institute of Oncology

Tool Details

  • Tool name: Anti-Cdc20 [AR12.4]
  • Alternate names: Fizzy protein, CDC2, CDC2A, p55CDC, P55CDC-LSB Human Entrez Gene ID 991 Human SwissProt Q12834 Human Unigene 524947 Human Gene Symbol CDC2 Human Chromosome Location 1p34.2
  • Clone: AR12.4
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Human
  • Host: Mouse
  • Molecular weight of the target: 55 kDa
  • Application: FACS ; IHC ; IF ; IP
  • Strain: Balb/c
  • Description: Cdc20 (fizzy protein) exhibits protein kinase activity in vitro and exists in a complex with both cyclin B and a protein homologous to p13SUC1. Cdc2 kinase is the active subunit of the M phase promoting factor (MPF) and the M phase-specific Histone H1 kinase. The p34Cdc2/cyclin B complex is required for the G2 to M transition. An additional cell cycle-dependent protein kinase, termed p55cdc, exhibits a high degree of homology with the S. cerevisiae proteins Cdc20 and Cdc4. The p55cdc transcript is readily detectable in a variety of cultured cell lines in growth phase, but disappears when cell growth is chemically arrested.
  • Immunogen: Urea-denatured His6 human Cdc20 recombinant protein
  • Isotype: IgG1 kappa
  • Research area: Cell biology; Biochemistry
  • Myeloma used: P3X63Ag8.653

  • For Research Use Only

Target Details

  • Target: Cell division cycle protein 20 (cdc20)
  • Target molecular weight: 55 kDa
  • Target background: Cdc20 (fizzy protein) exhibits protein kinase activity in vitro and exists in a complex with both cyclin B and a protein homologous to p13SUC1. Cdc2 kinase is the active subunit of the M phase promoting factor (MPF) and the M phase-specific Histone H1 kinase. The p34Cdc2/cyclin B complex is required for the G2 to M transition. An additional cell cycle-dependent protein kinase, termed p55cdc, exhibits a high degree of homology with the S. cerevisiae proteins Cdc20 and Cdc4. The p55cdc transcript is readily detectable in a variety of cultured cell lines in growth phase, but disappears when cell growth is chemically arrested.

Application Details

  • Application: FACS ; IHC ; IF ; IP

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: -15°C to -25°C
  • Shipping conditions: Shipping at 4°C

Documentation

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References

  • •  Sironi et al. 2001. EMBO J. 20(22):6371-82. PMID: 11707408.
  • •  Mad2 binding to Mad1 and Cdc20, rather than oligomerization, is required for the spindle checkpoint.