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Contributor Information

  • Name Walter Bodmer
  • Institute University of Oxford

Tool Details

  • Tool name: Anti-HLA-A2 [BB7.2]
  • Clone: BB7.2
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Human
  • Host: Mouse
  • Application: FACS ; IHC ; IF ; Fn
  • Strain: Balb/c
  • Description: Monoclonal antibody directed against HLA-A2 histocompatibility antigen, used to aid investigation into foreign antigen presentation and HLA mRNA production.
  • Immunogen: Papain solubilised HLA-A2,
  • Immunogen UniProt ID: P04439
  • Isotype: IgG2b
  • Research area: Immunology
  • Myeloma used: P3/NS1/1-Ag4.1

  • For Research Use Only

Target Details

  • Target: Human Leukocyte Antigen- A2
  • Target background: Human Leukocyte Antigens (HLA) are highly polymorphic proteins that are involved in the presentation of antigens to the T-cell receptor. There are two classes of HLA antigens, class I (HLA-A, HLA-B and HLA-C) and class II (HLA-D). The polymorphic HLA-A, -B, -C glycoproteins have a molecular weight of 43,000 daltons and are expressed on the surfaces of nearly all somatic cells, except red blood cells. This antibody can be used for HLA typing. Anti-HLA-A2 BB7.2 antibody recognizes human leukocyte antigen (HLA) A2 which is a subset of MHC-class I molecules encoded by the A.02 allele. It recognises an epitope at the C-terminus of alpha-2 helix and a turn on one of the underlying beta strands. Anti-HLA-A2 BB7.2 binds to an epitope at the C-terminus of the alpha-2 helix within the human HLA-A2 histocompatibility antigen. This antibody has exhibited blocking activity. Anti-HLA monoclonal antibodies can help identify in vitro translation production from HLA region mRNA, alongside isolating specific mRNA via precipitation or binding of polysomes. Anti-HLA-A2 can aid study within the immune response, including the presentation of foreign antigens to the immune system.

Application Details

  • Application: FACS ; IHC ; IF ; Fn

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: Store at -20°C frozen. Avoid repeated freeze / thaw cycles
  • Shipping conditions: Shipping at 4°C

Documentation

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References

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