ANTIBODIES
Contributor Information
- Name Julian Gannon
- Institute Cancer Research UK, London Research Institute: Clare Hall Laboratories
Tool Details
- Tool name: Anti-TK15 epitope tag [TK 15] mAb
- Clone: TK 15
- Tool type: Antibodies
- Tool sub-type: Primary antibody
- Class: Monoclonal
- Conjugate: Unconjugated
- Reactivity: Xenopus laevis
- Host: Mouse
- Application: IF ; IP ; WB
- Description: The anti-TK15 monoclonal antibody was raised against Xenopus laevis Orc1p and recognises recombinant proteins containing a single copy of an eight amino acid TK15 tag at the carboxy terminus. Amino terminal and internal TK15 tags have not been tested.
- Immunogen: Recombinant full length Xenopus Orc1p protein
- Isotype: IgG2a
- Research area: Cell biology; Tags and cell markers
- For Research Use Only
Target Details
- Target: TK15 epitope tag
- Target background: This is a new epitope tag antibody, developed for the purification and detection of recombinant fusion proteins incorporating a single eight amino acid TK15 tag.
Application Details
- Application: IF ; IP ; WB
Handling
- Format: Liquid
- Concentration: 1 mg/ml
- Storage buffer: PBS with 0.02% azide
- Storage conditions: -15° C to -25° C
- Shipping conditions: Shipping at 4° C
Documentation
Related Tools
References
- • Tugal et al. 1998. J Biol Chem. 273(49):32421-9. PMID: 9829972.
- • The Orc4p and Orc5p subunits of the Xenopus and human origin recognition complex are related to Orc1p and Cdc6p.
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