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Contributor Information

  • Name David Lane
  • Institute University of Dundee
  • Primary citation Vojtesek et al. 1992. J Immunol Methods. 151(1-2):237-44. PMID: 1378473

Tool Details

  • Tool name: Anti-p53 [Pab DO-7]
  • Clone: Pab DO-7
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Human
  • Host: Mouse
  • Cancer type: Broadly Applicable
  • Molecular weight of the target: 53 kDa
  • Application: ELISA ; FACS ; IHC ; IF ; WB
  • Description: A monoclonal p53 antibody, the epitope recognised by DO-7 is in the N terminus of p53. DO-7 can detect wild-type and mutant p53. DO-7 recognises three of the p53 isoforms (p53, p53β, p53Îł).
  • Immunogen: p53
  • Immunogen UniProt ID: P04637
  • Isotype: IgG2b
  • Research area: Apoptosis and autophagy; Cancer; Cell biology; Genetics

  • For Research Use Only

Target Details

  • Target: p53 (N terminus)
  • Target molecular weight: 53 kDa
  • Target background: p53 is a crucial tumour suppressor involved in over 50% of cancers. It acts as a stress-responsive transcription factor and plays a vital role in regulating cell cycle arrest, promoting apoptosis, maintaining genomic stability, controlling the cell cycle, and inhibiting angiogenesis. Known as the "guardian of the genome," p53 prevents gene mutations.

    Mutations in the p53 gene are common in human cancers, resulting in dysfunctional proteins unable to bind to DNA. This loss of function compromises p53's tumour suppressor activity. While p53 is typically found in low levels in healthy cells due to its short lifespan, disease states lead to increased expression driven by somatic mutations. This heightened expression is believed to contribute to cellular transformation and malignancy.

    Twelve isoforms of p53 have been described: p53α, p53β, p53γ, Δ40p53α, Δ40p53β, Δ40p53γ, Δ133p53α, Δ133p53β, Δ133p53γ, Δ160p53α, Δ160p53β, and Δ160p53γ. Depending on the location of the epitope, antibodies may detect different isoforms of p53. Mutations to p53 can cause conformational changes that expose different epitopes.

    This antibody was created by David Lane, who also discovered p53, to measure p53 in various analyses of different tissue samples. Anti-p53 DO-7 has been used frequently for staining fixed sections. DO-7 recognises an epitope in the N terminus region of p53. DO-7 recognises both wild-type and mutant p53. DO-7 recognises three of the p53 isoforms (p53, p53β, p53γ).

Application Details

  • Application: ELISA ; FACS ; IHC ; IF ; WB

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: Store at -20°C frozen. Avoid repeated freeze / thaw cycles
  • Shipping conditions: Shipping at 4°C

Documentation

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References

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  • •  Ilhan et al. 2016. J Vet Diagn Invest. PMID: 27016721.
  • •  Alsafadi et al. 2016. Oncotarget. 7(11):12331-43. PMID: 26575022.
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  • •  Ko et al. 2012. Pathol Int. 62(1):43-8. PMID: 22192803.
  • •  Molenaar et al. 2009. Proc Natl Acad Sci U S A. 106(31):12968-73. PMID: 19525400.
  • •  Stephen et al. 1995. J Mol Biol. 248(1):58-78. PMID: 7537340.
  • •  Vojtesek et al. 1992. J Immunol Methods. 151(1-2):237-44. PMID: 1378473.