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Contributor Information

  • Name Julian Gannon
  • Institute Cancer Research UK, London Research Institute: Clare Hall Laboratories

Tool Details

  • Tool name: Anti-CyclinA [AT10.2]
  • Alternate names: CCNA1; Cyclin A1; Testicular Tissue Protein Li 34; CT146
  • Clone: AT10.2
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Human
  • Host: Mouse
  • Application: IHC ; IP ; WB
  • Strain: Balb/c
  • Description: Cyclins bind to and regulate the activity of the Cyclin Dependent Protein Kinases (CDKs). Cyclin A is involved in the regulation of the cell cycle and is essential for progression through S phase. Cyclin A protein is absent in cells prior to S-phase, during which its levels increase and peak. Cyclin A is a marker for actively proliferating cells and for cells in S phase. E23.1 is extremely sensitive for immunoblotting Cyclin A.
  • Immunogen: Recombinant Human Cyclin A
  • Isotype: IgG1
  • Research area: Cancer; Cell biology; Genetics
  • Myeloma used: Sp2/0-Ag14

  • For Research Use Only

Target Details

  • Target: Cyclin A
  • Target background: Cyclins bind to and regulate the activity of the Cyclin Dependent Protein Kinases (CDKs). Cyclin A is involved in the regulation of the cell cycle and is essential for progression through S phase. Cyclin A protein is absent in cells prior to S-phase, during which its levels increase and peak. Cyclin A is a marker for actively proliferating cells and for cells in S phase.

Application Details

  • Application: IHC ; IP ; WB

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: -15°C to -25°C
  • Shipping conditions: Shipping at 4°C

Documentation

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References

  • •  Geley et al. 2001. J Cell Biol. 153(1):137-48. PMID: 11285280.
  • •  Anaphase-promoting complex/cyclosome-dependent proteolysis of human cyclin A starts at the beginning of mitosis and is not subject to the spindle assembly checkpoint.
  • •  Adamczewski et al. 1993. J Virol. 67(11):6551-7. PMID: 8411358.
  • •  Simian virus 40 large T antigen associates with cyclin A and p33cdk2.