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Contributor Information

  • Name Sharon Tooze
  • Institute Cancer Research UK, London Research Institute: Lincoln's Inn Fields

Tool Details

  • Tool name: Anti-ARF1 [ARFS 1A9/5]
  • Clone: ARFS 1A9/5
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Human
  • Host: Mouse
  • Application: WB ; ELISA ; IF ; WB
  • Strain: Balb/c
  • Description: ARF1 is a member of the ARF family of small G proteins and is a component of Golgi-derived non-clathrin coated vesicles.
  • Immunogen: C-terminal Human ARF1 peptide SNQLRNQ
  • Isotype: IgG2a
  • Research area: Cell signaling and signal transduction
  • Myeloma used: Sp2/0-Ag14

  • For Research Use Only

Target Details

  • Target: ADP-Ribosylation Factor 1 (ARF1)
  • Target background: ARF1 is a member of the ARF family of small G proteins and is a component of Golgi-derived non-clathrin coated vesicles.

Application Details

  • Application: WB ; ELISA ; IF ; WB

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: -15°C to -25°C
  • Shipping conditions: Shipping at 4°C

Documentation

References

  •   Ignashkova et al. 2017. Traffic. 18(8):530-544. PMID: 28485883.
  •   Cell survival and protein secretion associated with Golgi integrity in response to Golgi stress-inducing agents.
  •   Puxeddu et al. 2009. Proc Natl Acad Sci U S A. 106(15):6158-63. PMID: 19332778.
  •   Interaction of phosphodiesterase 3A with brefeldin A-inhibited guanine nucleotide-exchange proteins BIG1 and BIG2 and effect on ARF1 activity.
  •   Austin et al. 2000. J Biol Chem. 275(29):21862-9. PMID: 10807927.
  •   Direct and GTP-dependent interaction of ADP-ribosylation factor 1 with clathrin adaptor protein AP-1 on immature secretory granules.