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Contributor Information

  • Institute Cancer Research UK, London Research Institute: Lincoln's Inn Fields

Tool Details

  • Tool name: Anti-CDKN2A/p14ARF [ARF 4C6/4]
  • Clone: ARF 4C6/4
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Human
  • Host: Mouse
  • Molecular weight of the target: 14 kDa
  • Application: ELISA ; IHC ; IF ; IP ; WB
  • Strain: Balb/c
  • Description: p14ARF is associated with the regulation of p53 and MDM2 and is deleted or methylated in many human cancers. The gene for CDK2NA generates several transcripts/proteins which differ from each other in their first exons. Three of these transcripts are generated by alternative splicing (isoform 1 a.k.a p16INK4A, isoform 2 and isoform 3 a.k.a p12), two of which are known to function as inhibitors of CDK4 kinase. One other transcript that is generated from this gene contains an alternate reading frame (ARF), with the first exon located 20kb upstream of the remainder of the gene(isoform 4 a.k.a. p14ARF, p19ARF, ARF). In spite of the structural and some functional differences, all the proteins encoded by the CDKN2A gene are involved in cell cycle G1 control.
  • Immunogen: His-tagged p14ARF protein expressed in bacteria.
  • Isotype: IgG2a
  • Research area: Cancer; Cell biology; Genetics
  • Myeloma used: Sp2/0-Ag14

  • For Research Use Only

Target Details

  • Target: p14 ARF
  • Target molecular weight: 14 kDa
  • Target background: p14ARF is associated with the regulation of p53 and MDM2 and is deleted or methylated in many human cancers. The gene for CDK2NA generates several transcripts/proteins which differ from each other in their first exons. Three of these transcripts are generated by alternative splicing (isoform 1 a.k.a p16INK4A, isoform 2 and isoform 3 a.k.a p12), two of which are known to function as inhibitors of CDK4 kinase. One other transcript that is generated from this gene contains an alternate reading frame (ARF), with the first exon located 20kb upstream of the remainder of the gene(isoform 4 a.k.a. p14ARF, p19ARF, ARF). In spite of the structural and some Fn differences, all the proteins encoded by the CDKN2A gene are involved in cell cycle G1 control.

Application Details

  • Application: ELISA ; IHC ; IF ; IP ; WB

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: -15°C to -25°C
  • Shipping conditions: Shipping at 4°C

Documentation

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References

  • •  Ma et al. 2007. Mol Biol Cell. 18(7):2630-5. PMID: 17494866.
  • •  Depletion of the nucleolar protein nucleostemin causes G1 cell cycle arrest via the p53 pathway.
  • •  p14ARF expression in invasive breast cancers and ductal carcinoma in situ--relationships to p53 and Hdm2.
  • •  Vestey et al. 2004. Breast Cancer Res. 6(5):R571-85. PMID: 15318938.
  • •  Clark et al. 2002. Oncogene. 21(29):4498-507. PMID: 12085228.
  • •  Multiple interacting domains contribute to p14ARF mediated inhibition of MDM2.
  • •  Llanos et al. 2001. Nat Cell Biol. 3(5):445-52. PMID: 11331871.
  • •  Stabilization of p53 by p14ARF without relocation of MDM2 to the nucleolus.