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Contributor Information

  • Name Gerald Hart
  • Institute Johns Hopkins University

Tool Details

  • Tool name: Anti-O-GlcNAc (CTD110.6)
  • Tool type: Antibodies
  • Tool sub-type: Primary Antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Human ; Mouse ; Primate ; Rat ; All
  • Host: Mouse
  • Application: ELISA ; IHC ; IP ; WB
  • Description: The ?-O-linked N-acetylglucosamine (O-GlcNAc) antibody recognizes glycosylation, a post-translational modification, which occurs on serine and threonine residues of proteins in the nuclear and cytoplasmic compartments. This type of glycosylation results in a single moiety and not a complex branched structure. The O-GlcNAc antibody does not cross react with ?-linked Ser/Thr-O-GlcNAc, ?-linked Ser-O-linked N-acetylgalactosamine or N-linked oligosaccharides on ovalbumin and immunoglobulin G.
  • Immunogen: Synthetic glycopeptide (YSPTS(O-GlcNAc)PSK)
  • Isotype: IgM
  • Research area: Cell signaling and signal transduction; Biochemistry

  • For Research Use Only

Target Details

  • Target: ?-O-linked N-acetylglucosamine (O-GlcNAc) in ?-O-glycosidic linkage to both serine and threonine and does not cross react with a-linked Ser/Thr-O-GlcNAc, a-linked Ser-O-linked N-acetylgalactosamine or N-linked oligosaccharides on ovalbumin and immunoglobulin G.
  • Target background: The ?-O-linked N-acetylglucosamine (O-GlcNAc) antibody recognizes glycosylation, a post-translational modification, which occurs on serine and threonine residues of proteins in the nuclear and cytoplasmic compartments. This type of glycosylation results in a single moiety and not a complex branched structure. The O-GlcNAc antibody does not cross react with a-linked Ser/Thr-O-GlcNAc, a-linked Ser-O-linked N-acetylgalactosamine or N-linked oligosaccharides on ovalbumin and immunoglobulin G.

Application Details

  • Application: ELISA ; IHC ; IP ; WB

Handling

  • Format: Liquid
  • Storage conditions: -20°C
  • Shipping conditions: Shipping at 4°C

Documentation

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References

  • •  Comer et al. 2001. Anal Biochem. 293(2):169-77. PMID: 11399029.
  • •  Characterization of a mouse monoclonal antibody specific for O-linked N-acetylglucosamine.