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Contributor Information

  • Name Peter Parker
  • Institute Cancer Research UK, London Research Institute: Lincoln's Inn Fields

Tool Details

  • Tool name: Anti-FSBA [FSBA 2F5/5]
  • Clone: FSBA 2F5/5
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Host: Mouse
  • Application: IP ; WB ; ELISA ; IHC ; IF ; IP ; WB
  • Strain: Balb/c
  • Description: FSBA 2F5/5 has been shown to detect Protein Kinase C-?1 treated with FSBA.
  • Isotype: IgG
  • Research area: Cell biology; Tags and cell markers
  • Myeloma used: Sp2/0-Ag14

  • For Research Use Only

Target Details

  • Target: Fluorylsulponylbenzoyladenosine (FSBA)
  • Target background: FSBA, a reactive ATP analogue, can be used to selectively label protein kinases, which may subsequently be detected and affinity purified using an anti-FSBA antibody.

Application Details

  • Application: IP ; WB ; ELISA ; IHC ; IF ; IP ; WB

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: -15°C to -25°C
  • Shipping conditions: Shipping at 4°C

Documentation

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References

  • •  Poyurovsky et al. 2003. Mol Cell. 12(4):875-87. PMID: 14580339.
  • •  Nucleotide binding by the Mdm2 RING domain facilitates Arf-independent Mdm2 nucleolar localization.
  • •  Parker et al. 1993. FEBS Lett. 334(3):347-50. PMID: 8243645.
  • •  Antibodies to fluorylsulfonylbenzoyladenosine permit identification of protein kinases.