Product Image

Contributor Information

  • Name Paul Crocker
  • Institute University of Oxford

Tool Details

  • Tool name: Anti-CD33 [6C5/2]
  • Alternate names: Sialic Acid Binding Ig Like Lectin 12; SIGLECL1; SLG 3; Siglec-12; Siglec-L1; S2V
  • Clone: CD33 6C5/2
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Human
  • Host: Mouse
  • Application: ELISA ; FACS ; IHC
  • Description: The SIGLECs are a family of membrane bound lectins (of the immunoglobulin superfamily) that bind sialic acid and mediate cell-cell interactions. Family members include sialoadhesin, CD22 and CD33. CD33 is found on granulocyte and macrophage precursors in the bone marrow, but not on pluripotent stem cells. CD33 is also expressed on, and a useful marker for, peripheral monocytes. CD33 is also useful for distinguishing myelogenous leukaemia cells from lymphoid or erythroid leukaemias. CD33 is a putative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells & preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Induces apoptosis in acute myeloid leukemia (in vitro).
  • Immunogen: CD33-Fc recombinant protein
  • Isotype: IgG1
  • Research area: Cancer; Cell biology; Immunology; Stem cell biology
  • Myeloma used: Sp2/0-Ag14

  • For Research Use Only

Target Details

  • Target: SIGLEC3 (CD33)
  • Target background: The SIGLECs are a family of membrane bound lectins (of the immunoglobulin superfamily) that bind sialic acid and mediate cell-cell interactions. Family members include sialoadhesin, CD22 and CD33. CD33 is found on granulocyte and macrophage precursors in the bone marrow, but not on pluripotent stem cells. CD33 is also expressed on, and a useful marker for, peripheral monocytes. CD33 is also useful for distinguishing myelogenous leukaemia cells from lymphoid or erythroid leukaemias. CD33 is a putative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells & preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Induces apoptosis in acute myeloid leukemia (in vitro).

Application Details

  • Application: ELISA ; FACS ; IHC

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: -15°C to -25°C
  • Shipping conditions: Shipping at 4°C

Documentation

Related Tools

Product Image

Anti-VAChT (Whole Serum, Rabbit)

#160410
Product Image

Anti-PD-1 [J110]

#153513
Product Image

Anti-Activin C [BetaC1]

#153637

References

  • •  Souza et al. 2019. Short-Term High-Fat Diet Consumption Reduces Hypothalamic Expression of the Nicotinic Acetylcholine Receptor a7 Subunit (a7nAChR) and Affects the Anti-inflammatory Response in a Mouse Model of Sepsis Front Immunol. 10:565. PMID: 309678
  • •  Siglecs facilitate HIV-1 infection of macrophages through adhesion with viral sialic acids.
  • •  The myeloid-specific sialic acid-binding receptor, CD33, associates with the protein-tyrosine phosphatases, SHP-1 and SHP-2.
  • •  Characterization of CD33 as a new member of the sialoadhesin family of cellular interaction molecules.