ANTIBODIES

Contributor Information
- Name Stephen West
- Institute Cancer Research UK, London Research Institute: Clare Hall Laboratories
Tool Details
- Tool name: Anti-Rad51 [Rad51 3C10] mAb
- Clone: Rad51 3C10
- Tool type: Antibodies
- Tool sub-type: Primary antibody
- Class: Monoclonal
- Conjugate: Unconjugated
- Reactivity: Bovine ; Human ; Pig
- Host: Mouse
- Application: IHC ; IP ; WB
- Strain: Balb/c
- Description: Monoclonal antibody which binds RAD51 recombinase protein, associated with DNA repair pathway.
- Immunogen: Full length recombinant human RAD51 protein
- Immunogen UniProt ID: Q06609
- Isotype: IgG1
- Research area: Genetics
- Myeloma used: Sp2/0-Ag14
- For Research Use Only
Target Details
- Target: Rad51
- Target background: RAD51 is a eukaryotic homologue of E. coli RecA, a recombinase, and a component of the homologous recombination DNA repair pathway. RAD51 forms a nucleoprotein filament (through binding RAD52 and single stranded DNA that are exposed following double strand breaks) that initiates recombination. RAD51 catalyses the recognition of homology and strand exchange between homologous DNA partners to form a joint molecule between a processed DNA break and the repair template. Mutations in the gene can results in breast cancer, mirror movements 2 and Fanconi anaemia.
Application Details
- Application: IHC ; IP ; WB
Handling
- Format: Liquid
- Concentration: 1 mg/ml
- Storage buffer: PBS with 0.02% azide
- Storage conditions: Store at -20° C frozen. Avoid repeated freeze / thaw cycles
- Shipping conditions: Shipping at 4° C
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References
- • Vohhodina et al. 2017. Nucleic Acids Res. 45(22):12816-12833. PMID: 29112714.
- • Subramanyam et al. 2016. Proc Natl Acad Sci U S A. 113(41):E6045-E6054. PMID: 27671650.
- • Marampon et al. 2016. Oncotarget. 7(5):5383-400. PMID: 26689991.
- • Ivanov et al. 2003. J Cell Sci. 116(Pt 20):4095-106. PMID: 12953071.
- • Endopolyploid cells produced after severe genotoxic damage have the potential to repair DNA double strand breaks.
- • Masson et al. 1999. EMBO J. 18(22):6552-60. PMID: 10562567.
- • The meiosis-specific recombinase hDmc1 forms ring structures and interacts with hRad51.
- • Barlow et al. 1997. EMBO J. 16(17):5207-15. PMID: 9311981.
- • Distribution of the Rad51 recombinase in human and mouse spermatocytes.