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Contributor Information

  • Name Stephen Dilworth
  • Institute Cancer Research UK, London Research Institute: Lincoln's Inn Fields

Tool Details

  • Tool name: Anti-PyLT [PyLT]
  • Clone: PyLT
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Virus
  • Host: Rat
  • Molecular weight of the target: 100 kDa
  • Application: IF ; IP ; WB
  • Description: PyLT specifically detects and allows the isolation of the large T antigen.
  • Immunogen: Polyoma virus-transformed Wistar rat fibroblast cell line Py REWA5/T1A1.
  • Isotype: IgG2b
  • Research area: Microbiology
  • Myeloma used: P3/NS1/1-Ag4.1

  • For Research Use Only

Target Details

  • Target: Polyoma virus Large T antigen
  • Target molecular weight: 100 kDa
  • Target background: The mouse polyoma virus (Py) is an oncogenic virus, which encodes for three early proteins, large, middle and small T (tumour) antigen.

Application Details

  • Application: IF ; IP ; WB

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: -15°C to -25°C
  • Shipping conditions: Shipping at 4°C

Documentation

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References

  • •  Dilworth et al. 1982. EMBO J. 1(11):1319-28. PMID: 6327264.
  • •  Dilworth et al. 1982. Proc Natl Acad Sci U S A. 79(4):1059-63. PMID: 6175960.
  • •  Monoclonal antibodies against polyoma virus tumor antigens.
  • •  Protein kinase activities associated with distinct antigenic forms of polyoma virus middle T-antigen.