Product Image

Contributor Information

  • Name Peter Beverley
  • Institute Cancer Research UK, London Research Institute: Lincoln's Inn Fields

Tool Details

  • Tool name: Anti-CD3 monoclonal antibody [UCH-T1™]
  • Alternate names: T3 complex
  • Clone: UCH-T1
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Human
  • Host: Mouse
  • Application: FACS ; IHC ; IF ; IP ; Fn ; RIA ; WB
  • Strain: Balb/c
  • Description: CRT trademarked famous anti-CD3 monoclonal antibody, capable of differentiating between T vs B cells lymphomas and leukaemia's.
  • Immunogen: Human infant thymocytes and Sezary cells.
  • Immunogen UniProt ID: P04234
  • Isotype: IgG1
  • Research area: Cancer; Cell biology; Cell signaling and signal transduction; Immunology; Stem cell biology
  • Myeloma used: P3/NS1/1-Ag4.1
  • Additional notes: UCH-T1Ě˝is a registered trademark of Cancer Research Technology, Limited. All rights reserved.

  • For Research Use Only

Target Details

  • Target: CD3
  • Target background: The CD3 complex, composed of four distinct CD3 polypeptide chains (CD3?, CD3d and 2X CD3), associates with the T cell antigen receptor (TCR). It is found on all mature human T lymphocytes, NK cells and some thymocytes. CD3 is a member of the immunoglobulin superfamily, involved in antigen recognition, T lymphocyte activation and signal transduction. UCH-T1 is considered a pan T-cell marker - it can be used for the detection of T cell populations in peripheral blood and lymph nodes and the categorisation of T versus B cell lymphomas and leukaemia's. It reacts with the majority of peripheral blood T lymphocytes, a major proportion of thymocytes, the majority of T cell chronic lymphocytic leukaemia cells, Szary leukaemia's and approximately 70% of acute lymphoblastic leukaemia's of T cell origin. It can also be used to study the role of CD3 in TCR signal transduction events. This antibody was created by Professor Peter Beverley, a pioneer in creating hybridomas from mice immunised against human lymphocytes, with UCHT1 being one of the first successful fusions.

Application Details

  • Application: FACS ; IHC ; IF ; IP ; Fn ; RIA ; WB

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: Store at -20°C frozen. Avoid repeated freeze / thaw cycles
  • Shipping conditions: Shipping at 4°C

Documentation

Related Tools

Product Image

Anti-HLADQ3 [2HB6]

#151224
Product Image

Anti-Leu2 [C8/144B] rAb

#154829
Product Image

Anti-ORF38 [ORF38]

#160639

References

  • •  Bencsikova et al. 2019. BMC Cancer. 19(1):687. PMID: 31307428.
  • •  Oll Hurtado et al. 2019. PLoS One. 14(8):e0216373. PMID: 31398192.
  • •  Acquaviva et al. 2019. Front Immunol. 10:1922. PMID: 31474991.
  • •  rsek et al. 2012. J Immunol. 189(4):1602-10. PMID: 22798681.
  • •  CD3-chain expression of human T lymphocytes is regulated by TNF via Src-like adaptor protein-dependent proteasomal degradation.
  • •  Muhammad et al. 2009. J Immunol. 182(12):7672-80. PMID: 19494291.
  • •  Sequential cooperation of CD2 and CD48 in the buildup of the early TCR signalosome.
  • •  Nishi et al. 2008. Glycobiology. 18(12):1065-73. PMID: 18796645.
  • •  Fn and structural bases of a cysteine-less mutant as a long-lasting substitute for galectin-1.
  • •  Dornan et al. 2002. J Biol Chem. 277(3):1912-8. PMID: 11694532.
  • •  Differential association of CD45 isoforms with CD4 and CD8 regulates the actions of specific pools of p56lck tyrosine kinase in T cell antigen receptor signal transduction.
  • •  Lafont et al. 1999. J Biol Chem. 274(36):25743-8. PMID: 10464312.
  • •  Evidence for a p21(ras)/Raf-1/MEK-1/ERK-2-independent pathway in stimulation of IL-2 gene transcription in human primary T lymphocytes.
  • •  Salmern et al. 1991. J Immunol. 147(9):3047-52. PMID: 1717585.
  • •  A conformational epitope expressed upon association of CD3-epsilon with either CD3-delta or CD3-gamma is the main target for recognition by anti-CD3 monoclonal antibodies.
  • •  Kanellopoulos et al. 1983. EMBO J. 2(10):1807-14. PMID: 6227478.
  • •  Biosynthesis and molecular nature of the T3 antigen of human T lymphocytes.
  • •  Garson et al. 1982. Nature. 298(5872):375-7. PMID: 6178042.
  • •  Monoclonal antibodies against human T lymphocytes label Purkinje neurones of many species.
  • •  Beverley et al. 1981. Eur J Immunol. 11(4):329-34. PMID: 6788570.
  • •  Distinctive Fn characteristics of human "T" lymphocytes defined by E rosetting or a monoclonal anti-T cell antibody.