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Contributor Information

  • Name Peter Beverley
  • Institute Cancer Research UK, London Research Institute: Lincoln's Inn Fields

Tool Details

  • Tool name: Anti-LewisX [TG-1]
  • Alternate names: Fucosyltransferase 4; Galactoside 3-L-Fucosyltransferase; ELAM-1 Ligand Fucosyltransferase; FUC-TIV; FCT3A; ELFT; Stage-Specific Embryonic Antigen; Alpha (1,3) Fucosyltransferase; EC 2.4.1.65; Lewis X; SSEA-1; FUTIV; CD15; LeX
  • Clone: TG-1
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Human
  • Host: Mouse
  • Molecular weight of the target: 156 kDa
  • Application: FACS ; IHC ; IF
  • Strain: CBA
  • Description: TG-1 is useful for the differentiation of human leukocytes.
  • Immunogen: Thymocyte glycoprotein fraction adsorbed to Con A and eluted with 0.1M glucose.
  • Isotype: IgM
  • Research area: Cancer; Immunology
  • Myeloma used: P3/NS1/1-Ag4.1

  • For Research Use Only

Target Details

  • Target: Lewis X (CD15)
  • Target molecular weight: 156 kDa
  • Target background: Lewis X (CD15) is a branched pentasaccharide found on neutrophils, eosinophils and monocytes. Lewis X is distributed abnormally in myeloid leukeamias and is commonly used in the diagnosis of Hodgkin's disease.

Application Details

  • Application: FACS ; IHC ; IF

Handling

  • Format: Liquid
  • Concentration: 0.9-1.1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: -15°C to -25°C
  • Shipping conditions: Shipping at 4°C

Documentation

References

  •   Cho et al. 2010. J Proteome Res. 9(11):5960-8. PMID: 20858014.
  •   Sialylated Lewis x antigen bearing glycoproteins in human plasma.
  •   Hogg et al. 1984. Immunology. 53(4):753-67. PMID: 6389324.
  •   Monoclonal antibodies specific for human monocytes, granulocytes and endothelium.
  •   Linch et al. 1984. Blood. 63(3):566-73. PMID: 6365201.
  •   Monoclonal antibodies differentiating between monocytic and nonmonocytic variants of AML.
  •   Beverley et al. 1980. Nature. 287(5780):332-3. PMID: 7421991.
  •   Isolation of human haematopoietic progenitor cells using monoclonal antibodies.