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Contributor Information

  • Name Peter Parker
  • Institute Cancer Research UK, London Research Institute: Lincoln's Inn Fields

Tool Details

  • Tool name: Anti-PKCa [MC5]
  • Clone: MC5
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Bovine
  • Host: Mouse
  • Molecular weight of the target: 80 kDa
  • Application: ELISA ; FACS ; IHC ; IF ; IP ; RIA ; WB
  • Strain: Balb/c
  • Description: MC5 blocks partial proteolysis of PKC by trypsin in vitro. Fab fragment inhibits phorbol-ester-induced down-regulation of PKC in a rodent gloma cell line.
  • Immunogen: Purified bovine brain protein kinase C
  • Isotype: IgG2a
  • Research area: Cancer; Tissue-specific biology; Cell signaling and signal transduction
  • Myeloma used: P3X63Ag8

  • For Research Use Only

Target Details

  • Target: Protein Kinase C alpha
  • Target molecular weight: 80 kDa
  • Target background: Protein Kinase C is a family of serine/threonine protein kinases that are activated by Ca2+, diacylglycerol and membrane phospholipids (phosphatidylserine).

Application Details

  • Application: ELISA ; FACS ; IHC ; IF ; IP ; RIA ; WB

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: -15°C to -25°C
  • Shipping conditions: Shipping at 4°C

Documentation

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References

  • •  Kim et al. 2008. Nature. 452(7186):478-82. PMID: 18368118.
  • •  Molecular identification of a retinal cell type that responds to upward motion.
  • •  Ng et al. 1999. EMBO J. 18(14):3909-23. PMID: 10406796.
  • •  PKCalpha regulates beta1 integrin-dependent cell motility through association and control of integrin traffic.
  • •  Young et al. 1988. Eur J Biochem. 173(1):247-52. PMID: 2451608.
  • •  A monoclonal antibody recognising the site of limited proteolysis of protein kinase C. Inhibition of down-regulation in vivo.