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Contributor Information

  • Name John Kemshead
  • Institute Institute of Child Health

Tool Details

  • Tool name: Anti-Vimentin [J144]
  • Clone: J144
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Human
  • Host: Mouse
  • Application: IHC ; IF ; IP ; WB
  • Description: J144 may be used in the staining of intracellular proteins to determine tissue of origin.
  • Immunogen: Human rhabdosarcoma cell line JR1
  • Isotype: IgM
  • Research area: Cancer; Cell biology; Cell signaling and signal transduction; Developmental biology; Neurobiology

  • For Research Use Only

Target Details

  • Target: Vimentin
  • Target background: Vimentin is an intermediate filament protein, which is expressed in most cells of mesodermal origin but not epithelial cells.

Application Details

  • Application: IHC ; IF ; IP ; WB

Handling

  • Format: Liquid
  • Concentration: 0.9-1.1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: -15°C to -25°C
  • Shipping conditions: Shipping at 4°C

Documentation

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References

  • •  Doldo et al. 2015. Genes Cancer. 6(11-12):490-502. PMID: 26807202.
  • •  High expression of cellular retinol binding protein-1 in lung adenocarcinoma is associated with poor prognosis.
  • •  Dubicke et al. 2008. Mol Hum Reprod. 14(11):641-7. PMID: 18922847.
  • •  Different secretion patterns of matrix metalloproteinases and IL-8 and effect of corticotropin-releasing hormone in preterm and term cervical fibroblasts.
  • •  Oppedal et al. 1987. Histopathology. 11(4):363-74. PMID: 3596475.
  • •  Immunohistochemical differentiation of neuroblastomas from other small round cell neoplasms of childhood using a panel of mono- and polyclonal antibodies.