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Contributor Information

  • Name Walter Bodmer
  • Institute Cancer Research UK, London Research Institute: Lincoln's Inn Fields

Tool Details

  • Tool name: Anti-PLAP [HD 11F7]
  • Clone: HD 11F7
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Human
  • Host: Mouse
  • Application: ELISA ; IHC ; RIA
  • Strain: Balb/c
  • Description: Monoclonal antibody with potential use in detection of ovarian and testicular tumours via detection of PLAP.
  • Immunogen: Fresh Hep-2 cells followed by booster injections of fresh Hep-2 cells that had their surface expression of PLAP boosted in culture.
  • Immunogen UniProt ID: P05187
  • Isotype: IgG2b
  • Research area: Cancer; Cell biology
  • Myeloma used: P3/NS1/1-Ag4.1

  • For Research Use Only

Target Details

  • Target: Placental alkaline phosphatase (PLAP)
  • Target background: Human placental alkaline phosphatase (PLAP) is a membrane bound glycosylated phosphodiesterase normally synthesised by syncytiotrophoblast from the 12th week of pregnancy. Since its identification as an oncofoetal antigen, it has been found to be expressed by malignant tumours of germ cell and non-germ cell origin. The detection of alkaline phosphatase in serum is a marker for ovarian and testicular cancer. HD 11F7 has potential uses in the diagnosis of ovarian and testicular tumours. This antibody detects both Regan and Nagao isoenzymes. The placental-specific isozyme of Alkaline Phosphatase (PLAP), also referred to as the heat-stable form, is found in trophoblast cells of normal human mature placenta, seminomas of testis and ovarian carcinomas. It is closely related to the intestinal form of the enzyme as well as to the placental-like form. Anti-PLAP was created for use in immunoscintigraphy or therapy.

Application Details

  • Application: ELISA ; IHC ; RIA

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: Store at -20°C frozen. Avoid repeated freeze / thaw cycles
  • Shipping conditions: Shipping at 4°C

Documentation

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References

  • •  Fitzgerald et al. 2018. Am J Reprod Immunol. 80(1):e12860. PMID: 29726582.
  • •  Durbin et al. 1988. Int J Cancer Suppl. 2:59-66. PMID: 3162446.
  • •  Monoclonal antibodies to placental alkaline phosphatase: preclinical evaluation in a human xenograft tumour model of F(ab')2 and Fab fragments.