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Contributor Information

  • Name Joyce Taylor-Papadimitriou
  • Institute Cancer Research UK, London Research Institute: Lincoln's Inn Fields

Tool Details

  • Tool name: Anti-Fibronectin [Fn-3]
  • Clone: Fn-3
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Human
  • Host: Mouse
  • Application: IHC ; IF ; IP ; WB
  • Strain: Balb/c
  • Description: Monoclonal antibody capable of differentiating between cellular and plasma fibronectin, in healthy cells or tumours.
  • Immunogen: FR5 cells, derived by SV40 transformation of human mammary epithelial cells.
  • Immunogen UniProt ID: P02751
  • Isotype: IgG1
  • Research area: Cell biology; Cancer; Tissue-specific biology; Cell signaling and signal transduction; Developmental biology; Stem cell biology
  • Myeloma used: P3X63Ag8.653

  • For Research Use Only

Target Details

  • Target: Fibronectin
  • Target background: Fibronectin is a widely expressed extracellular matrix glycoprotein that is exists in two forms, a soluble plasma form and a less soluble cellular form. Fibronectin is involved in cell adhesion, maintenance of cell shape and migration processes including embryogenesis, wound healing, blood coagulation, host defence, and metastasis. Cellular fibronectin is often found in the stroma of malignant tumours. Fn-3 can distinguish between human cellular and plasma fibronectin, thus making it possible to analyse the origin and levels of different types of fibronectin in normal human tissues and their related tumours.

Application Details

  • Application: IHC ; IF ; IP ; WB

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: Store at -20°C frozen. Avoid repeated freeze / thaw cycles
  • Shipping conditions: Shipping at 4°C

Documentation

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References

  • •  Bambang et al. 2013. Oncogene. 32(10):1240-51. PMID: 22543584.
  • •  Endoplasmic reticulum protein 29 regulates epithelial cell integrity during the mesenchymal-epithelial transition in breast cancer cells.
  • •  Cseh et al. 2010. J Cell Sci. 123(Pt 22):3989-99. PMID: 20980391.
  • •  Autocrine fibronectin directs matrix assembly and crosstalk between cell-matrix and cell-cell adhesion in vascular endothelial cells.
  • •  Keen et al. 1984. Mol Biol Med. 2(1):15-27. PMID: 6083431.
  • •  Monoclonal antibodies that distinguish between human cellular and plasma fibronectin.