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Contributor Information

  • Name Mike Horton
  • Institute Queen Mary University of London

Tool Details

  • Tool name: Anti-Integrin b3 [F11]
  • Clone: F11
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Human ; Rat
  • Host: Mouse
  • Application: FACS ; IHC ; IP ; Fn
  • Strain: Balb/c
  • Description: The F11 epitope is different to the MHF4 epitope.
  • Immunogen: A bone cell suspension containing osteoclasts, osteoblasts and bone marrow cells obtained from the long bones of newborn Sprague Dawley rats.
  • Isotype: IgG1
  • Research area: Cell biology; Tissue-specific biology; Cell signaling and signal transduction
  • Myeloma used: P3X63Ag8.653

  • For Research Use Only

Target Details

  • Target: Integrin ?3 (CD61)
  • Target background: Integrins are heterodimeric cell surface receptors composed of alpha and beta subunits, which mediate cell-cell and cell-extracellular matrix attachments. Aberrant integrin expression has been found in many epithelial tumours. Changes in integrin expression have been shown to be important for the growth and early metastatic capacity of melanoma cells. Integrin b3 is expressed in osteoclasts, megacaryocytes and platelets.

Application Details

  • Application: FACS ; IHC ; IP ; Fn

Handling

  • Format: Liquid
  • Concentration: 0.9-1.1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: -15°C to -25°C
  • Shipping conditions: Shipping at 4°C

Documentation

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References

  • •  de Nigris et al. 2007. Oncogene. 26(3):382-94. PMID: 16878156.
  • •  Cooperation between Myc and YY1 provides novel silencing transcriptional targets of alpha3beta1-integrin in tumour cells.
  • •  Thompson et al. 2000. J Immunol. 165(1):426-34. PMID: 10861081.
  • •  Divergent effects of platelet-endothelial cell adhesion molecule-1 and beta 3 integrin blockade on leukocyte transmigration in vivo.
  • •  Shankar et al. 1993. J Cell Sci. 105 ( Pt 1):61-8. PMID: 7689577.
  • •  Integrin receptor-mediated mobilisation of intranuclear calcium in rat osteoclasts.
  • •  Helfrich et al. 1992. J Bone Miner Res. 7(3):345-51. PMID: 1585837.
  • •  Helfrich et al. 1992. J Bone Miner Res. 7(3):335-43. PMID: 1585836.
  • •  Integrins on rat osteoclasts: characterization of two monoclonal antibodies (F4 and F11) to rat beta 3.
  • •  Rat osteoclasts adhere to a wide range of RGD (Arg-Gly-Asp) peptide-containing proteins, including the bone sialoproteins and fibronectin, via a beta 3 integrin.