ANTIBODIES
Contributor Information
- Name Walter Bodmer
- Institute Cancer Research UK, London Research Institute: Lincoln's Inn Fields
Tool Details
- Tool name: Anti-Alkaline phosphatase [AAP1]
- Clone: AAP1
- Tool type: Antibodies
- Tool sub-type: Primary antibody
- Class: Monoclonal
- Conjugate: Unconjugated
- Reactivity: Human
- Host: Mouse
- Application: WB ; ELISA
- Strain: Balb/c
- Description: Alkaline phosphatases are phosphodiesterases which remove phosphate groups from the 5' end of DNA, RNA and proteins at high pH. The placental-specific isozyme of Alkaline Phosphatase (PLAP) is found in trophoblast cells of normal human mature placenta, seminomas of testis and ovarian carcinomas. Detection of alkaline phosphatase in serum is a marker for ovarian and testicular cancer.
- Immunogen: D98/AH-2 (HeLa) cells, which produce human intestinal alkaline phosphatase ectopically
- Isotype: IgG2a
- Research area: Cancer; Cell biology
- Myeloma used: P3X63Ag8
- For Research Use Only
Target Details
- Target: Alkaline phosphatase
- Target background: Alkaline phosphatases are phosphodiesterases which remove phosphate groups from the 5' end of DNA, RNA and proteins at high pH. The placental-specific isozyme of Alkaline Phosphatase (PLAP) is found in trophoblast cells of normal human mature placenta, seminomas of testis and ovarian carcinomas. Detection of alkaline phosphatase in serum is a marker for ovarian and testicular cancer.
Application Details
- Application: WB ; ELISA
Handling
- Format: Liquid
- Concentration: 1 mg/ml
- Storage buffer: PBS with 0.02% azide
- Storage conditions: -15Ă°C to -25Ă°C
- Shipping conditions: Shipping at 4Ă°C
Documentation
Related Tools
References
- • Brock et al. 1985. Lancet. 1(8439):1175-8. PMID: 2860384.
- • Prospective prenatal diagnosis of cystic fibrosis.
- • Arklie et al. 1981. Tissue Antigens. 17(3):303-12. PMID: 7314066.
- • A monoclonal antibody to intestinal alkaline phosphatase made against D98/AH-2 (HeLa) cells.
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