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Contributor Information

  • Institute Cancer Research UK, London Research Institute: Lincoln's Inn Fields

Tool Details

  • Tool name: Anti-ERCC1 [ERCC1 3H11]
  • Clone: ERCC1 3H11
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Human
  • Host: Mouse
  • Molecular weight of the target: 33-36
  • Application: IF ; IP ; WB
  • Strain: Balb/c
  • Description: Nucleotide excision repair (NER) is a DNA repair pathway that removes lesions induced by a variety of agents such as UV irradiation. ERCC1 binds to XPF to form the heterodimer ERCC1-XPF, which is a DNA endonuclease that is essential for the dual incision step of NER (cleaves 5' of the DNA lesion). Some publications report that ERCC1 is elevated in cisplatin-resistant cells.
  • Immunogen: Full length HIS-tagged recombinant human ERCC1
  • Isotype: IgG2a
  • Research area: Cancer; Genetics
  • Myeloma used: Sp2/0-Ag14

  • For Research Use Only

Target Details

  • Target: ERCC1
  • Target molecular weight: 33-36
  • Target background: Nucleotide excision repair (NER) is a DNA repair pathway that removes lesions induced by a variety of agents such as UV irradiation. ERCC1 binds to XPF to form the heterodimer ERCC1-XPF, which is a DNA endonuclease that is essential for the dual incision step of NER (cleaves 5' of the DNA lesion). Some publications report that ERCC1 is elevated in cisplatin-resistant cells.

Application Details

  • Application: IF ; IP ; WB

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: -15°C to -25°C
  • Shipping conditions: Shipping at 4°C

Documentation

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References

  • •  HCMV-infected cells maintain efficient nucleotide excision repair of the viral genome while abrogating repair of the host genome.
  • •  O'Dowd et al. 2012. PLoS Pathog. 8(11):e1003038. PMID: 23209410.
  • •  Bhagwat et al. 2009. Cancer Res. 69(17):6831-8. PMID: 19723666.
  • •  Immunodetection of DNA repair endonuclease ERCC1-XPF in human tissue.
  • •  Biggerstaff et al. 1993. EMBO J. 12(9):3685-92. PMID: 8253090.
  • •  Co-correction of the ERCC1, ERCC4 and xeroderma pigmentosum group F DNA repair defects in vitro.