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Contributor Information

  • Name Gerard Evan
  • Institute Absolute Antibody ; University of California, San Francisco
  • Primary citation Evan et al. 1985. Mol Cell Biol. 5(12):3610-6. PMID: 3915782.

Tool Details

  • Tool name: Anti-cMyc recombinant antibody [9E10]
  • Clone: r9E10
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Recombinant
  • Conjugate: Unconjugated
  • Reactivity: Human
  • Host: Mouse
  • Cancer type: Gynaecologic cancer; Digestive / Gastrointestinal cancer; Breast cancer; Lung; Blood cancer
  • Application: WB
  • Description: Recombinant monoclonal antibody raised against sequence of c-Myc, a proto-oncogene heavily implicated in a variety of (hematopoietic) cancers. The anti-c-Myc antibody is a recombinant version of the monoclonal antibody 9E10, specifically designed to bind to p62 and detect the c-Myc gene product in human cells. It is particularly useful in immunoblots for quantifying c-Myc levels. This recombinant 9E10 antibody can recognize recombinant proteins carrying a c-Myc epitope tag and detect human c-Myc protein.
  • Immunogen: Synthetic peptide (AEEQKLISEEDLLRKRREQLKHKLEQLRNSCA - 32 aa) corresponding to 408-439 of Human c-Myc conjugated to KLH.
  • Immunogen UniProt ID: P01106
  • Isotype: IgG1 kappa
  • Research area: Cancer; Cell biology; Genetics; Stem cell biology

  • For Research Use Only

Target Details

  • Target: c Myc epitope tag
  • Target background: The anti-c-Myc antibody specifically targets the proto-oncogene c-Myc, which is frequently upregulated in cancer. Overexpression of c-Myc is known to contribute to the activation of genes involved in cellular proliferation and has been observed in various cancers, including cervical, colon, breast, lung, stomach, and Burkitt's lymphoma resulting from translocation. The gene product of c-Myc, known as p62, is upregulated in several hematopoietic tumours. Given that c-Myc is believed to regulate around 15% of all genes, it plays a significant role in cancer development and is considered a promising therapeutic target.

    c-Myc plays a crucial role in apoptosis, cell cycle progression, proliferation, and differentiation. Its activities, both beneficial and oncogenic, primarily occur within the nucleus. The c-Myc protein, a 62 kDa transcription factor, is encoded by the c-Myc gene located on human chromosome 8q24.

    The anti-c-Myc antibody is a recombinant version of the monoclonal antibody 9E10, specifically designed to bind to p62 and detect the c-Myc gene product in human cells. It is particularly useful in immunoblots for quantifying c-Myc levels. This recombinant 9E10 antibody can recognize recombinant proteins carrying a c-Myc epitope tag and detect human c-Myc protein, as well as a peptide in a random coil configuration rather than a helix.

Application Details

  • Application: WB

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Storage buffer: PBS only
  • Storage conditions: -15°C to -25°C
  • Shipping conditions: Shipping at 4°C

Documentation

References

  •   Munro and Pelham. 1987. Cell. 13:48(5):899-907. PMID: 3545499.
  •   Munro and Pelham. 1986 Cell. 18:46(2):291-300. PMID: 3087629.
  •   Evan et al. 1985. Mol Cell Biol. 5(12):3610-6. PMID: 3915782.