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Contributor Information

  • Name Rosemonde Banks
  • Institute University of Leeds

Tool Details

  • Tool name: Anti-IL12 [1-1A4]
  • Alternate names: Interleukin 12; Cytotoxic Lymphocyte Maturation Factor 4 KDa Subunit; NK Cell Stimulatory Factor Chain; Interleukin-12 Beta Chain; IL-12 Subunit P4; CLMF P4; NKSF2; Natural Killer Cell Stimulatory Factor; IL12; Subunit P4; Interleukin 12; CLMF2; IMD29; NKSF; P4
  • Clone: 1-1A4
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Human
  • Host: Mouse
  • Application: IHC ; ELISA ; IHC ; RIA ; WB
  • Description: Monoclonal antibody which binds p40 subunit of interleukin-12.
  • Immunogen: Recombinant human IL-12 produced in Baculovirus.
  • Immunogen UniProt ID: P29459
  • Isotype: IgG1
  • Research area: Immunology
  • Myeloma used: Sp2/0-Ag14

  • For Research Use Only

Target Details

  • Target: Interleukin-12 (IL12) p40 subunit
  • Target background: IL-12 is a heterodimeric cytokine comprised of p35 and p40 subunits. IL-12 plays a central role in cell-mediated immunity, promoting the differentiation of CD4+ T cells to the Th1 subset and of CD8+ T cells into mature cytotoxic T lymphocytes (CTLs). Interleukin 12 (IL-12) is produced by dendritic cells, macrophages, neutrophils, and human B-lymphoblastoid cells in response to antigenic stimulation. IL-12 is a potent stimulator of Natural Killer cells. IL-12 therapy has been suggested as a method of enhancing cytocidal anti-tumour immune responses. This antibody recognises the p40 subunit of human IL-12 and binds to both the free subunit and the p35/p40 heterodimer. It reacts with both stimulated and unstimulated monocytes in immunohistology.

Application Details

  • Application: IHC ; ELISA ; IHC ; RIA ; WB

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: Store at -20°C frozen. Avoid repeated freeze / thaw cycles
  • Shipping conditions: Shipping at 4°C

Documentation

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References

  • •  Nagashima et al. 2008. Anticancer Res. 28(2B):1277-83. PMID: 18505066.
  • •  Prognostic significance of the local expression of interleukin-12 in patients with advanced gastric cancer.
  • •  Nakayama et al. 2004. Anticancer Res. 24(5C):3289-94. PMID: 15515423.
  • •  Relationships between local and systemic expression of interleukin-12 and plasma levels of vascular endothelial growth factor in patients with gastric cancer.
  • •  Carter et al. 1997. Hybridoma. 16(4):363-9. PMID: 9309427.
  • •  Production and characterization of monoclonal antibodies to human interleukin-12.