Product Image

Contributor Information

  • Name Alison Goodall
  • Institute University College London (UCL)

Tool Details

  • Tool name: Anti-von Willebrand Factor [RFF-VIII:R/1]
  • Clone: RFF-VIII:R/1
  • Tool type: Antibodies
  • Tool sub-type: Primary Antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Primate
  • Host: Mouse
  • Application: ELISA ; FACS ; IF ; Fn ; RIA ; WB
  • Description: Anti-Anti-Von Willebrand Factor [RFF-VIII:R/1] specifically binds to human factor VIII related antigen (VIII:RAg) in plasma and in vascular endothelial cells but has no reactivity with factor VIII procoagulant antigen (VIII:cAg). This antibody is a potent inhibitor of von Willebrand factor activity in that it can totally neutralise risocetin-induced aggregation of platelet rich plasma and inhibit platelet adhesion at high flow rates. RFF-VIII:R/2 can be used in a one-stage, fluid phase immunoradiometric assay that can detect VIII:RAg. This monoclonal antibody binds to human von Willebrand factor (VW) and inhibits it's binding to platelets. Very similar in characteristics to RFF-VIII:R/1.
  • Immunogen: Purified human Factor VIII-VWF complex
  • Isotype: IgG1 kappa
  • Research area: Tissue-specific biology; Stem cell biology
  • Myeloma used: P3/NS1/1-Ag4.1

  • For Research Use Only

Target Details

  • Target: Von Willebrand factor (VWF)
  • Target background: Anti-Anti-Von Willebrand Factor [RFF-VIII:R/1] specifically binds to human factor VIII related antigen (VIII:RAg) in plasma and in vascular endothelial cells but has no reactivity with factor VIII procoagulant antigen (VIII:cAg). This antibody is a potent inhibitor of von Willebrand factor activity in that it can totally neutralise risocetin-induced aggregation of platelet rich plasma and inhibit platelet adhesion at high flow rates. RFF-VIII:R/2 can be used in a one-stage, fluid phase immunoradiometric assay that can detect VIII:RAg. This monoclonal antibody binds to human von Willebrand factor (VW) and inhibits it's binding to platelets. Very similar in characteristics to RFF-VIII:R/1.

Application Details

  • Application: ELISA ; FACS ; IF ; Fn ; RIA ; WB

Handling

  • Format: Liquid
  • Shipping conditions: Shipping at 4°C

Documentation

Related Tools

Product Image

Anti-HLAD/DR, DQ [35]

#151046
Product Image

Anti-Plet1 [33A10]

#154797
Product Image

Anti-OxPL [mAB-509]

#160858

References

  • •  [Use of RFF-VIII:R/1 in the purification of FVIII]
  • •  Murdock et al. 1997. Thromb Haemost. 78(4):1272-7. PMID: 9364997.
  • •  von Willebrand factor activity detected in a monoclonal antibody-based ELISA: an alternative to the ristocetin cofactor platelet agglutination assay for diagnostic use.
  • •  Takase et al. 1987. Br J Haematol. 66(4):497-502. PMID: 3117089.
  • •  Production of factor VIII deficient plasma by immunodepletion using three monoclonal antibodies.
  • •  McCraw et al. 1987. Thromb Res. 45(1):101-8. PMID: 3494325.
  • •  A monoclonal antibody based immunoradiometric assay for von Willebrand factor: survey of a large patient group.
  • •  Chand et al. 1986. Thromb Haemost. 55(3):318-24. PMID: 2428124.
  • •  A two-site, monoclonal antibody-based immunoassay for von Willebrand factor--demonstration that vWF function resides in a conformational epitope.
  • •  Rotblat et al. 1985. Biochemistry. 24(16):4294-300. PMID: 2413885.
  • •  Purification of human factor VIII:C and its characterization by WBting using monoclonal antibodies.
  • •  Goodall et al. 1985. Br J Haematol. 59(4):565-77. PMID: 2580547.
  • •  An immunoradiometric assay for human factor VIII/von Willebrand factor (VIII:vWF) using a monoclonal antibody that defines a Fn epitope.