How are the monoclonal antibodies produced?
By in vitro production methods; we do not produce in ascites fluid.
How are the monoclonal antibodies purified?
IgG by Protein A (murine) or Protein G (rat) affinity chromatography, which typically give a > 95% degree of purity. IgM is purified using a thiophilic chromatographic method.
How is the antibody concentration determined?
By UV absorption at 280 nm, in a 1 cm path length cuvette using an extinction coefficient of 1.4.
Are bovine serum supplements used?
Yes, from USFDA approved serum suppliers.
How are the antibodies stored and dispatched?
They are stored at -20°C and dispatched at ambient temperature in padded jiffy bags containing cool packs.
Can monoclonal antibodies be supplied in other formulations such as, “bulk” size or without sodium azide?
If you do have individual requirements, we are more than happy to discuss them so please do not hesitate to contact us
Are there any recommended dilutions for usage?
These depend on the intended application and must be determined individually.
What are the storage conditions for monoclonal antibodies upon receipt?
Most antibodies are quite robust and should retain functional activity if kept refrigerated at 2—8°C for up to 12 months. For longer term storage, it is recommended to store the material frozen in smaller aliquots. Avoid repeated freezing and thawing cycles, which will adversely affect the material.
Due to the large inventory size, individual stability studies have not been conducted on every antibody.
How are monoclonal antibodies supplied?
Monoclonal antibodies are supplied as 1.1 ml aliquots (to include overage) at a concentration of either 1.0mg/ml or 2.0mg/mL ±0.1mg/mL, in Phosphate Buffered Saline (PBS) containing 0.02% sodium azide as preservative.
CAUTION: Sodium azide is toxic if ingested and due care should be exercised.
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