Product Image

Contributor Information

  • Name Mikhail Plentnikov
  • Institute Johns Hopkins University

Tool Details

  • Tool name: Mutant DISC1 Inducible Transgenic mouse
  • Tool type: Experimental models
  • Tool sub-type: Mouse
  • Disease: Schizophrenia
  • Model: Transgenic
  • Model description:
  • Genetic background and cross history: B6;SJL-Tg(TRE-CMV-hDISC1) crossed with B6;CBA-Tg(Camk2a-tTA)1Mmay/j mice
  • Description: A strong candidate gene for schizophrenia and major mental disorders, disrupted-in-schizophrenia 1 (DISC1) has been implicated in neurodevelopment, including maturation of the cerebral cortex. Translocation mutation may result in loss of DISC1 function via haploinsufficiency or dominant-negative effects of a predicted mutant DISC1 truncated protein product. Transgenic mice with inducible expression of mutant human DISC1 (hDISC1) limited to forebrain regions, including cerebral cortex, hippocampus and striatum were generated using the TET-off gene expression system under the regulation of the CAMKII promoter. Expression of mutant hDISC1 was not shown to be associated with gross neurodevelopmental abnormalities, but did produce mild abnormalities. Compared to their sex-matched littermate controls, mutant hDISC1 transgenic male mice exhibited spontaneous hyperactivity in the open field and alterations in social interaction, and transgenic female mice showed deficient spatial memory. Neuronal and behavioral effects of mutant hDISC1 are consistent with a dominant-negative mechanism, and are similar to some features of schizophrenia.
  • Research area: Drug development; Neurobiology

  • For Research Use Only

Target Details

  • Target: DISC1

Application Details

Handling

Documentation

Related Tools

Product Image

MMTV TET-LRH-1 Transgenic Mouse

#153997
Product Image

ATF2 Mouse

#151648
Product Image

Tsc1iDeltaEC Tumor model for lymphangiosarcoma mouse

#160865

References

  • •  Pletnikov et al. 2008. Mol Psychiatry. 13(2):173-86, 115. PMID: 17848917.