Product Image

Contributor Information

  • Name Laura Machesky
  • Institute Cancer Research UK, Glasgow: The Beatson Institute

Tool Details

  • Tool name: LifeAct-GFP Mouse
  • Alternate names: Hypoxanthine Phosphoribosyltransferase 1, HGPRTase, HGPRT, HPRT, F-actin
  • Tool type: Experimental models
  • Tool sub-type: Mouse
  • Model: Reporter
  • Conditional: Yes
  • Conditional description: Expression of Cre recombinase excises the lox stop lox cassette encoded upstream of the LifeAct-GFP marker enabling its expression.
  • Zygosity: Heterozygous
  • Strain: C57BL/6
  • Description: This Cre-inducible transgenic mouse can conditionally express cell-type specific LifeAct-GFP. This is possible due to a floxed neomycin resistance 'STOP' cassette encoded upstream of the LifeAct-GFP fusion cDNA in the Hprt locus of the mouse X chromosome. LifeAct is a 17 amino acid peptide, isolated from yeast, which facilitates visualisation of filamentous actin (F-actin) in cells and tissues without significantly interfering with actin dynamics in vitro or in vivo. This enables live imaging of the actin cytoskeleton and therefore the study of many fundamental biological processes like cytokinesis. Incorporation of LifeAct in this specific and selective manner allows the study of actin dynamics in cell types, particularly primary cells, which are not readily transfectable. It also permits the study of intracellular actin in cells whilst present in their native environment. The ability to visualise actin in this way surpasses other reagents used to visualize the actin cytoskeleton like fluorescent phalloidin and anti-actin antibodies.
  • Research area: Cell biology; Genetics

  • For Research Use Only

Target Details

  • Target: Actin cytoskeleton

Application Details

Handling

  • Shipping conditions: Embryo/Spermatoza- Dry Ice

Documentation

Related Tools

Product Image

UNG Mouse

#151556
Product Image

Siglec-1 (CD169) KO mouse

#154180
Product Image

CYFIP 1 [10bp insertion] Zebrafish

#158358

References

  • •  Schachtner et al. 2012. Eur J Cell Biol. 91(11-12):923-9. PMID: 22658956.
  • •  Tissue inducible Lifeact expression allows visualization of actin dynamics in vivo and ex vivo.