EXPERIMENTAL MODELS
Contributor Information
- Name Catrin Pritchard
- Institute University of Leicester
Tool Details
- Tool name: LSLBRafD594A Mouse
- Tool type: Experimental models
- Tool sub-type: Mouse
- Cancer type: Gynaecologic cancer
- Disease: Cancer
- Conditional: Yes
- Conditional description: Cre-lox
- Genetic background and cross history: A transgenic vector was generated and used to modify the endogenous gene in E14.1a embryonic stem cells by homologous recombination. Targeted ES cells were microinjected into C57BL6 blastocysts and chimaeras were derived. These were bred to C57BL6 mice to obtain germline transmission, from which a colony of heterozygous LSL-BrafD594A mice on the C57BL6 background were derived.
- Phenotype: Progressive aneuploidy
- Zygosity:
- Description: Mimics the expression of D594ABRAF in human cancer samples. In conjunction with oncogenic G12DKRAS, this mutant induces the development of highly aggressive malignant melanomas. Conditional knockin (Cre-lox) D594A mutation of BRaf
- Research area: Cancer; Genetics
- Production details: A transgenic vector was generated and used to modify the endogenous gene in E14.1a embryonic stem cells by homologous recombination. Targeted ES cells were microinjected into C57BL6 blastocysts and chimaeras were derived. These were bred to C57BL6 mice to obtain germline transmission, from which a colony of heterozygous LSL-BrafD594A mice on the C57BL6 background were derived.
- Additional notes:
- For Research Use Only
Target Details
- Target: Mouse BRAF
Application Details
Handling
- Shipping conditions: Embryo/Spermatoza- Dry Ice
Documentation
References
- • Heidorn et al. 2010. Cell. 140(2):209-21. PMID: 20141835.
- • Kinase-dead BRAF and oncogenic RAS cooperate to drive tumor progression through CRAF.
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