CELL LINES
Contributor Information
- Name Julian Downward, Pablo Romero-Clavijo, Miriam Molina-Arcas
- Institute The Francis-Crick Institute
- Primary citation Boumelha et al. 2022. Cancer Research. 82(19):3435-3448. PMID: 35930804.
Tool Details
- Tool name: KPAR-G12C Cell Line
- Alternate names:
- Tool type: Cell Lines
- Organism: Mouse
- Tissue: Lung
- Cancer type: Lung cancer
- Model: Mutant
- Model description: A derivative from the KPAR cells, where the mutation Asp in position 12 from KRAS (G12D) was mutated to Cys (G12C) using prime editing.
- CRISPR: No
- Description: Mouse models of mutant KRAS-driven lung cancer with an elevated tumour mutational burden by expressing the human DNA cytosine deaminase, APOBEC3B, to mimic the mutational signature seen in human lung cancer. The ability of KRAS/KRASG12 inhibitors to cause regression of KRASG12C expressing tumours was markedly potentiated by the adaptive immune system.
- Research area: Cancer
- Production details: A derivative from the KPAR cells, where the mutation Asp in position 12 from KRAS (G12D) was mutated to Cys (G12C) using prime editing.
- For Research Use Only
Target Details
Application Details
Handling
- Growth medium: DMEM media supported with 10% FBS, glutamine and penicillin/streptomycin
- Storage conditions: Liquid Nitrogen
- Shipping conditions: Dry ice
- Cultured in antibiotics?: Penicillin, Streptomycin
- Biosafety level: CL-1
Documentation
Related Tools
References
- • Boumelha et al. 2022. Cancer Research. 82(19):3435-3448. PMID: 35930804.
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