CELL LINES
Contributor Information
- Name Pi-Ling Chang and Yu-Hua Hsieh
- Institute The UAB Research Foundation
- Primary citation Hsieh et.al. 2012. Int J Cancer. PMID: 21826648
Tool Details
- Tool name: Osteopontin null squamous cell carcinoma cell line
- Tool type: Cell Lines
- Organism: Mouse
- Donor: Female OPN-null mouse subjected to two-stage skin carcinogenesis
- Tissue: Skin
- Gender: Female
- Cancer type: Skin cancer
- Growth properties: Adherent
- CRISPR: No
- Receptors of note: No
- Description: Cells derived from a female mouse with cutaneous squamous cell carcinoma (SCC) lacking osteopontin (OPN) gene. Cells contain p53 and H-ras mutations which are common characteristics of SCCs. These cells develop SCC in syngeneic wild-type and OPN-null mice. Cell line is great for studying host-derived OPN at the systemic and tumor microenvironmnet level in the context of extrinsic cancer cell progression in immune-competent mice
- Research area: Cancer
- Production details: OPN-null and WT mice in 129S6/SvEv background were bred in-house. ONSC cells were derived from an SCC developed in a female OPN-null mouse subjected to two-stage skin carcinogenesis. Cells were maintained in high-glucose, low-calcium DMEM containing 10% fetal bovine serum.
- For Research Use Only
Target Details
Application Details
Handling
- Growth medium: DMEM with 10% fetal bovine serum
Documentation
Related Tools
References
- • Hsieh et.al. 2012. Int J Cancer. PMID: 21826648
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