CELL LINES
Contributor Information
- Name Steven Carroll
- Institute The UAB Research Foundation
- Primary citation Longo et. al. 2021. Sci Rep 11, 5690. PMID: 33707600
Tool Details
- Tool name: 2XSB Cell Line
- Alternate names: 2XSB Cell Line
- Tool type: Cell Lines
- Organism: Human
- Donor: 57 year old Caucasian woman with no previous history of cancer
- Tissue: Neoplasm from right brachial nerve (WHO grade IV sporadic MPNST)
- Gender: Female
- Cancer type: Sarcoma
- Morphology: Spindled to polygonal morphology with multiple cytoplasmic vacuoles. Subpopulation of cells that were larger and multinucleated also present.
- Growth properties: Adherent
- CRISPR: No
- Receptors of note: No
- Application: Karyotyping, STR profiling, ICC, IHC, Live cell imaging, TMEM, SNP, whole exome sequencing, TERT promoter Sanger seq, Western blot, and Ras activation assays.
- Description: Cell line derived from human with malignant peripheral nerve sheath tumors (MPNSTs). Cell line shares molecular and genomic features of parent tumor and is able to form solid tumors when xenografted into immunodeficient mice. 2XSB cells have functional NF1 alleles and no mutations in genes encoding the Polycomb Repressor Complex 2. Mutations in TP53 and PTEN were detected as well as a homozygous deletion of CDKN2A which aregenes implicated in MPNST pathogenesis. Other mutations included DNMT1, NUMA1, NTRK1, PDE11A, CSMD3, LRP5, and ACTL9 which are associated with the pathogenesis of other human cancers.
- Research area: Cancer
- Production details: Established from fresh tumor tissue that was placed in clulture plates with DMEM supplementeed with fetal calf serum, Schwann cell mitogen, forskolin, glutamin, streptomycin, and penicillin. Tissue was minced and allowed to migrate out of tissue for 72 hours. Afterwards, non-dispersed tissue was rinsed with PBS and trypsinized. DMEM and fetal calf serum was added and cells were centrifuged, resuspended, and added to cells that had previously migrated. Culture was llowed to grow until confluent with fresh media every 3-4 days addded. Confluent plates were trypsinized and split 1:2 and after five passages, nneuregulin-1B and forskolin were removed from media and cells were maintained in DMEM with fetal calf serum, glutamine, streptocycin, and penicillin.
- For Research Use Only
Target Details
Application Details
- Application: Karyotyping, STR profiling, ICC, IHC, Live cell imaging, TMEM, SNP, whole exome sequencing, TERT promoter Sanger seq, Western blot, and Ras activation assays.
Handling
- Growth medium: DMEM, 10% fetal calf serum, 10nM NRG1B, 2uM forskolin, 1% glutamine, 10ug/mL streptomycin, and 10IU/mL penicillin
- Temperature: 37C
- Atmosphere: 5% CO2
- Cultured in antibiotics?: Streptomycin and Penicillin
Documentation
Related Tools
References
- • Longo et. al. 2021. Sci Rep 11, 5690. PMID: 33707600
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