CELL LINES
Contributor Information
- Name Anne-Marie Mes-Masson and Diane Provencher
- Institute Centre Hospitalier de Luniversité de Montréal
- Primary citation Fleury et al. 2015. Genes & Cancer. 6(9-10):378-398. PMID: 26622941
Tool Details
- Tool name: OV-4453 cell line
- Alternate names: OV-4453
- Tool type: Cell Lines
- Organism: Human
- Donor: Age of diagnosis: 70; BRCA hereditary status: BRCA2(+); Stage IIIC; Mutations: BRCA2, TP53, CSMD2, RB1; Pre-treatment
- Tissue: Derived from ascites from patient with high grade serous carcinoma
- Gender: Female
- Cancer type: Gynaecologic cancer
- Morphology: Formed numerous individual small aggregates
- Growth properties: Adherent
- CRISPR: No
- Receptors of note: No
- Description: Epithelial ovarian cancer cell lines spontaneously derived from high-grade serious ascites from patient that had no chemotherapy prior to surgery. Patient received radiotherapy and hormonotherapy for breast cancer five year prior to ovarian cancer diagnosis.
- Research area: Cancer
- Production details: Established from the cellular fraction of ascites collected by centrifugation. The cell lines were then maintained for 40 days in OSE with medium replaced weekly.
- Additional notes: Patient 4453 was age 70 at diagnosis, BRCA hereditary status was BRCA2(+), and had previous history of breast cancer. Treatment for ovarian cancer included Carboplatin/taxol and interval surgery. Year of sampling was 2009.
- For Research Use Only
Target Details
Application Details
Handling
- Growth medium: OSE medium contains 10% FBS, 0.5ug/mL amphotericin B and 50 ug/mL gentamicin
- Atmosphere: Low oxygen conditions of 7% O2 and 5% CO2
- Cultured in antibiotics?: Amphotericin B and Gentamicin
Documentation
Related Tools
References
- • Vias et al. 2023. Elife. 11
- • 12:e83867. PMID: 37166279 Sevinyan et al. 2022. Cancers (Basel). 16
- • 14(22):5628. PMID: 36428724 Asare-Werehene et al. 2023. Cancers (Basel). 30
- • 15(9):2566. PMID: 37174032
.svg)
.svg)
