CELL LINES
Contributor Information
- Name Anne-Marie Mes-Masson and Diane Provencher
- Institute Centre Hospitalier de Luniversité de Montréal
- Primary citation Fleury et al. 2015. Genes & Cancer. 6(9-10):378-398. PMID: 26622941
Tool Details
- Tool name: TOV-3291G cell line
- Alternate names: TOV-3291G
- Tool type: Cell Lines
- Organism: Human
- Donor: Age of diagnosis: 59; BRCA hereditary status: negative Grade 3 Stage IIIC; Mutations: TP53, CDK12; Pre-treatment
- Tissue: Derived from high-grade serous tumors
- Gender: Female
- Cancer type: Gynaecologic cancer
- Morphology: Formed loosely compact spheroids with irregular margins
- Growth properties: Adherent
- CRISPR: No
- Receptors of note: No
- Description: Epithelial ovarian cancer cell lines spontaneously derived from high-grade serous solid tumors. TOV3291G harbors a TP53 mutation, a comlex CNA pattern, and has a CDK12 mutation.
- Research area: Cancer
- Production details: Established using the Scrape method where tumor tissue was scraped into a 100mm plate with complete OSE medium and maintained for 40 days with medium replaced weekly.
- Additional notes: Patient 3291 was age 59 at diagnosis and BRCA hereditary status was negative. Their first line treatment was surgery followed by cisplatin/taxol. No previous personal history of cancer and yera of sampling was 2007.
- For Research Use Only
Target Details
Application Details
Handling
- Growth medium: OSE medium contains 10% FBS, 0.5ug/mL amphotericin B and 50 ug/mL gentamicin
- Atmosphere: Low oxygen conditions of 7% O2 and 5% CO2
- Cultured in antibiotics?: Amphotericin B and Gentamicin
Documentation
Related Tools
References
- • Vias et al. 2023. Elife. 11
- • 12:e83867. PMID: 37166279 Sevinyan et al. 2022. Cancers (Basel). 16
- • 14(22):5628. PMID: 36428724 Asare-Werehene et al. 2023. Cancers (Basel). 30
- • 15(9):2566. PMID: 37174032
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