CELL LINES
Contributor Information
- Name Anne-Marie Mes-Masson and Diane Provencher
- Institute Centre Hospitalier de Luniversité de Montréal
- Primary citation Létourneau et al. 2012. BMC Cancer. (12): 379. PMID: 22931248
Tool Details
- Tool name: OV-3133(R2) cell line
- Alternate names: OV-3133(R2)
- Tool type: Cell Lines
- Organism: Human
- Donor: Grade 3 Stage IIIC; Mutations: TP53 Exon 6; Carboplatin/Taxol/Doxorubicin & Carboplatin/Gemcitabine/Doxorubicin
- Tissue: Derived from ascites of patient diagnosed with high-grade serous ovarian cancer at specific time points at diagnosis and relapse
- Gender: Female
- Cancer type: Gynaecologic cancer
- Morphology: No expression of the tumor suppressor p53 detected. Strong HER2 expression detected in protein extracts and observed in solid tissues by IHC. Spheroid formation: aggregate
- Growth properties: Adherent
- CRISPR: No
- Receptors of note: No
- Description: Epithelial ovarian cancer cell lines spontaneously derived from ascites of patient diagnosed with high-grade serous ovarian cancer at specific time points at diagnosis and relapse (R). Primary treatment included paclitaxel and carboplatin and later treatment included doxorubicin, carboplatin, gemcitabine, and etoposide.
- Research area: Cancer
- Production details: Established from the cellular fraction of ascites collected by centrifugation. The cell lines were then maintained for 40 days in OSE with medium replaced weekly.
- Additional notes: Patient 3133 received treatment of paclitaxel and carboplatin 1-3 months after sugery and confirmation of ovarian cancer diagnosis. A second sugery 6 months after the first detectede infiltration of the tumor in part of the abdomen. Patient was treated with dxorubicin and later carboplatin and gemcitabine after no significant changes of CA-125 levels were seen. Later imaging presented an increase in tumor mass whereby she was given etoposide orally for eight days.
- For Research Use Only
Target Details
Application Details
Handling
- Growth medium: OSE medium contains 10% FBS, 0.5ug/mL amphotericin B and 50 ug/mL gentamicin
- Atmosphere: Hypoxic condition of 5%O2 and 5%CO2
- Cultured in antibiotics?: Amphotericin B and Gentamicin
Documentation
Related Tools
References
- • Tomas et al. 2023. J Ovarian Res. 11
- • 16(1):70. PMID: 37038202 Vias et al. 2023. Elife. 11
- • 12:e83867. PMID: 37166279 Jubelin et al. 2022. Cell Biosci. 11
- • 12(1):155. PMID: 36089610 Canals et al. 2022. Front Oncol. 4
- • 12:856424. PMID: 35600398
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