CELL LINES
Contributor Information
- Name Anne-Marie Mes-Masson and Diane Provencher
- Institute Centre Hospitalier de Luniversité de Montréal
- Primary citation Létourneau et al. 2012. BMC Cancer. (12): 379. PMID: 22931248
Tool Details
- Tool name: OV-3133(R) cell line
- Alternate names: OV-3133(R)
- Tool type: Cell Lines
- Organism: Human
- Donor: Grade 3 Stage IIIC; Mutations: TP53 Exon 6; Carboplatin/Taxol
- Tissue: Derived from ascites of patient diagnosed with high-grade serous ovarian cancer at specific time points at diagnosis and relapse
- Gender: Female
- Cancer type: Gynaecologic cancer
- Morphology: Formed tumors at subcutaneous sites in SCID mice. No expressionof the tumor suppressor p53 detected. Strong HER2 expression detected in protein extracts and observed in solid tissues by IHC. Spheroid formation: no
- Growth properties: Adherent
- CRISPR: No
- Receptors of note: No
- Description: Epithelial ovarian cancer cell lines spontaneously derived from ascites of patient diagnosed with high-grade serous ovarian cancer at specific time points at diagnosis and relapse (R). Primary treatment included paclitaxel and carboplatin and later treatment included doxorubicin, carboplatin, gemcitabine, and etoposide.
- Research area: Cancer
- Production details: Established from the cellular fraction of ascites collected by centrifugation. The cell lines were then maintained for 40 days in OSE with medium replaced weekly.
- Additional notes: Patient 3133 received treatment of paclitaxel and carboplatin 1-3 months after sugery and confirmation of ovarian cancer diagnosis. A second sugery 6 months after the first detectede infiltration of the tumor in part of the abdomen. Patient was treated with dxorubicin and later carboplatin and gemcitabine after no significant changes of CA-125 levels were seen. Later imaging presented an increase in tumor mass whereby she was given etoposide orally for eight days.
- For Research Use Only
Target Details
Application Details
Handling
- Growth medium: OSE medium contains 10% FBS, 0.5ug/mL amphotericin B and 50 ug/mL gentamicin
- Atmosphere: Hypoxic condition of 5%O2 and 5%CO2
- Cultured in antibiotics?: Amphotericin B and Gentamicin
Documentation
Related Tools
References
- • Tomas et al. 2023. J Ovarian Res. 11
- • 16(1):70. PMID: 37038202 Vias et al. 2023. Elife. 11
- • 12:e83867. PMID: 37166279 Jubelin et al. 2022. Cell Biosci. 11
- • 12(1):155. PMID: 36089610 Canals et al. 2022. Front Oncol. 4
- • 12:856424. PMID: 35600398
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