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Contributor Information

  • Name Anne-Marie Mes-Masson and Diane Provencher
  • Institute Centre Hospitalier de L’université de Montréal
  • Primary citation Sauriol A et al. 2020. Cancers. 12(8):2222. PMID: 32784519

Tool Details

  • Tool name: TOV-2414 cell line
  • Alternate names: TOV-2414
  • Tool type: Cell Lines
  • Organism: Human
  • Donor: Grade 1 – Stage IIIC; Mutations: K-RAS2; Pre-treatment
  • Tissue: Derived from solid tumor from patient of mucinous carcinoma
  • Gender: Female
  • Cancer type: Gynaecologic cancer
  • Morphology: Stretched-like morphology
  • Growth properties: Adherent
  • CRISPR: No
  • Receptors of note: No
  • Description: Epithelial ovarian cancer cell line derived from solid tumor from patient of mucinous carcinoma who was chemonaive at the time of sampling. Cell line produces tumors in mice upon SC injection
  • Research area: Cancer
  • Production details: Established using the Scrape method where tumor tissue was scraped into a 100mm plate with complete OSE medium and maintained for 40 days with medium replaced weekly.
  • Additional notes: Patient 2414 was diagnosed at age 63 and had no prior cancer history. Site of sampling was the ovary and year of sampling was 2005.

  • For Research Use Only

Target Details

Application Details

Handling

  • Growth medium: OSE medium contains 10% FBS, 0.5ug/mL amphotericin B and 50 ug/mL gentamicin
  • Temperature: 37C
  • Atmosphere: Low oxygen conditions of 7% O2 and 5% CO2
  • Cultured in antibiotics?: Amphotericin B and Gentamicin

Documentation

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References

  • •  Gaia-Oltean et al. 2021. Exp Ther Med. 21(3):243. PMID: 33603851 Canals Hernaez et al. 2020. J Immunother Cancer. 8(2):e001128. PMID: 33243933 Borzdzi?owska et al. 2022. Biomedicines. 11
  • •  10(5):1116. PMID: 35625852 Meng et al. 2023. J Anim Sci. 3
  • •  101:skad215. PMID: 37351870 Koni et al. 2020. Int J Mol Sci. 18
  • •  21(20):7697. PMID: 33080952