CELL LINES
Contributor Information
- Name Anne-Marie Mes-Masson and Diane Provencher
- Institute Centre Hospitalier de Luniversité de Montréal
- Primary citation Sauriol A et al. 2020. Cancers. 12(8):2222. PMID: 32784519
Tool Details
- Tool name: OV-2085 cell line
- Alternate names: OV-2085
- Tool type: Cell Lines
- Organism: Human
- Donor: Grade 3 Stage IIIC;Mutations: TP53; Carboplatin/Taxol
- Tissue: Derived from ascites of patient with high grade serous carcinoma
- Gender: Female
- Cancer type: Gynaecologic cancer
- Morphology: Homogenous morphology after 60 passages - small clusters of loosely attached round cells growing perpendicularly to culture plate
- Growth properties: Adherent
- CRISPR: No
- Receptors of note: No
- Description: Epthelial ovarian cancer cell line derived from high grade serous carcinoma ascites. Cell line produces tumors in mice upon SC injection
- Research area: Cancer
- Production details: Patient ascites were centrifuged and seeded in 100mm plate with OSE medium and maintained for 40 days with weekly media replacement.
- Additional notes: Patient 2085 had family history of breast cancer, no BRCA mutation, and was age 63 at age of diagnosis. Patient received carboplatin and Paclitaxel prior to sampling. Sampling took place in 2004
- For Research Use Only
Target Details
Application Details
Handling
- Growth medium: OSE medium contains 10% FBS, 0.5ug/mL amphotericin B and 50 ug/mL gentamicin
- Temperature: 37C
- Atmosphere: Low oxygen conditions of 7% O2 and 5% CO2
- Cultured in antibiotics?: Amphotericin B and Gentamicin
Documentation
Related Tools
References
- • Gaia-Oltean et al. 2021. Exp Ther Med. 21(3):243. PMID: 33603851 Canals Hernaez et al. 2020. J Immunother Cancer. 8(2):e001128. PMID: 33243933 Borzdzi?owska et al. 2022. Biomedicines. 11
- • 10(5):1116. PMID: 35625852 Meng et al. 2023. J Anim Sci. 3
- • 101:skad215. PMID: 37351870 Koni et al. 2020. Int J Mol Sci. 18
- • 21(20):7697. PMID: 33080952
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