Product Image

Contributor Information

  • Name Anne-Marie Mes-Masson and Diane Provencher
  • Institute Centre Hospitalier de L’université de Montréal
  • Primary citation Ouellet et al. 2008. BMC Cancer. (8):152. PMID: 18507861

Tool Details

  • Tool name: OV-1946 cell line
  • Alternate names: OV-1946
  • Tool type: Cell Lines
  • Organism: Human
  • Donor: Grade 3 – Stage IIIC; Mutations: TP53 Exon 8; Pre-treatment
  • Tissue: Derived from matched ascites from case TOV-1946
  • Gender: Female
  • Cancer type: Gynaecologic cancer
  • Morphology: Able to form less compact spheroids and expressed Krt7
  • Growth properties: Adherent
  • CRISPR: No
  • Receptors of note: No
  • Description: Epithelial ovarian cancer cell line derived from matched ascites from TOV-1946. Cell line derived from ascites of chemotherapy naive patient who presented poorly differentiated (grade 3) serous papillary cystadenocarcinoma at stage IIC
  • Research area: Cancer
  • Production details: Established from a mass of cells from ascites of patient 1946. Mass was macro-dissected and kept in 100mm petri dish for 27 days where they reached 80% confluence. Tissue was discarded and cells were divided 2:3 every week for the first 15 passages and 1:2 twice a week until passage 70. Cells were mantianed and divided 1:5 twice a week afterwards
  • Additional notes: Patient 1946 had no known familial history of cancer and passed from post-operative complications

  • For Research Use Only

Target Details

Application Details

Handling

  • Growth medium: OSE medium contains 10% FBS, 0.5ug/mL amphotericin B and 50 ug/mL gentamicin
  • Atmosphere: Hypoxic condition of 5%O2 and 5%CO2
  • Cultured in antibiotics?: Amphotericin B and Gentamicin

Documentation

Related Tools

Product Image

MCF7/LetR-2 Cell Line

#152548
Product Image

Bovine Type II Alveolar Epithelial (B2AE) cell line

#157737
Product Image

BICR 78 Cell Line

#152849

References

  • •  Sauriol et al. 2023. Sci Rep. 27
  • •  13(1):3334. PMID: 36849518 Sevinyan et al. 2022. Cancers (Basel). 16
  • •  14(22):5628. PMID: 36428724 Brodeur et al. 2021. Sci Rep. 14
  • •  11(1):18183. PMID: 34521878 Communal et al. 2021. Int J Mol Sci. 18
  • •  22(10):5325. PMID: 34070214 Patra et al. 2020 PLoS One. 31
  • •  15(12):e0244549. PMID: 33382759