CELL LINES
Contributor Information
- Name Corrado Santocanale
- Institute National University of Ireland Galway
- Primary citation Rainey et al. 2017. ACS Chem Biol. 12(7):1893-1902. PMID: 28560864
Tool Details
- Tool name: MCF10A EditR (Constitutive Cas9 expression) cell line
- Tool type: Cell line
- Parental cell line: MCF10A
- Organism: Human
- Tissue: Breast
- Gender: Female
- Disease: Fibrocystic disease
- Growth properties: Adherent
- Model description: CRISPR
- CRISPR: Yes
- Products or characteristics of interest: CRISPR drug screening
- Application: SDS-PAGE and western blotting
- Description: MCF10AEditR cells that stably express Cas9 nuclease. The cell line is used to perform a CRISPR-Cas9 genome-wide screen to identify genes that, when lost, promote the proliferation of cells treated with sub-efficacious doses of a CDC7i
- Research area: Cancer
- Production details: MCF10AEditR cells that stably express Cas9 nuclease were generated by lentiviral transduction of the Edit-R Cas9 plasmid and blasticidin-mediated selection.
- For Research Use Only
Target Details
Application Details
- Application: SDS-PAGE and western blotting
Handling
- Growth medium: DMEM supplemented with 5% Horse serum, 100 ng/ml cholera toxin, 10 ?g/ml insulin, 20 ng/ml epidermal growth factor (Peprotech), 500 ng/ml hydrocortisone, 50 U/ml penicillin and 50 ?g/ml Streptomycin
- Temperature: 37 °C
- Atmosphere: 5% CO2
- Volume: Frozen
- Storage conditions: Liquid Nitrogen
- Shipping conditions: Dry ice
- Cultured in antibiotics?: Penicillin / streptomycin
- Biosafety level: 1
Documentation
Related Tools
References
- • Rainey et al. 2017. ACS Chem Biol. 12(7):1893-1902. PMID: 28560864
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