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Contributor Information

  • Name Martin Clynes
  • Institute Dublin City University
  • Primary citation Clary et. al. Biochemical Pharmacology, 1997 53(10): 1493-1502. PMID: 9260877

Tool Details

  • Tool name: DLKP-A10 cell line
  • Tool type: Cell line
  • Parental cell line: DLKP cell line
  • Organism: Human
  • Tissue: Lung
  • Cancer type: Lung cancer
  • Disease: Cancer
  • Morphology: Monolayer
  • CRISPR: No
  • Products or characteristics of interest: The cell line exhibits various levels of resistance to chemotherapeutic agents
  • Description: DLKP-A10 is a drug-resistant subpopulation of DLKP squamous carcinoma cell line. It overexpresses the MDR associated P-glycoprotein efflux pump.
  • Research area: Cancer
  • Production details: DLKP-A was established by continuous exposure of the DLKP cells (pDLKP) to escalating concentrations of Adr (Step 1: 0.05 ?g/mL–0.8 ?g/mL Adr for 11 months, Step 2: 0.8 ?g/mL–1.75 ?g/mL for 3 months and Step 3: 1.75 ?g/mL–2.1 ?g/mL for 3 months)

  • For Research Use Only

Target Details

Application Details

Handling

  • Growth medium: DMEM-F12 medium supplemented with 5% FBS and 1% L-glutamine
  • Temperature: 37 °C
  • Atmosphere: 5% CO2
  • Volume: Frozen
  • Storage conditions: Liquid Nitrogen
  • Shipping conditions: Dry ice
  • Mycoplasma free: Yes

Documentation

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References

  • •  Clary et. al. Biochemical Pharmacology, 1997 53(10): 1493-1502. PMID: 9260877
  • •  Efeoglu et. al. Biomolecules. 2022 Oct 1,12(10):1401. PMID: 36291610