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Contributor Information

  • Name Yvan Canitrot
  • Institute Toulouse Tech Transfer
  • Primary citation Fages et al. 2020. PLoS Genet 16(6):e e1008511. PMID: 32598339.

Tool Details

  • Tool name: Catalytically-inactive-JMJD6 U2OS cell line
  • Alternate names: JMJD6 KO+Mut
  • Tool type: Cell Lines
  • Tool sub-type: Continuous
  • Parental cell line: U2OS
  • Organism: Human
  • Tissue: Bone
  • Gender: Female
  • Cancer type: Sarcoma
  • Disease: Myasthenic Syndrome, Congenital, 9, Associated With Acetylcholine Receptor Deficiency and Pancreatitis
  • Morphology: Epithelial
  • Growth properties: Adherent
  • Model: Knock-Out; Knock-In
  • Model description: JMJD6 knock out using CRISPR-Cas9 and knock in of a catalytically inactive form of JMJD6
  • CRISPR: Yes
  • Conditional: No
  • Receptors of note: Insulin like growth factor
  • Application: Western blot, Immunofluorescence
  • Description: Catalitically inactive JMJD6 U2OS cell line used to study the role of the JMJD6 enzyme in DNA maintenance following DNA damage
  • Research area: Cancer
  • Production details: Gene inactivation by CRISPR-Cas9 and reintroduction of a mutant form of JMJD6 defective for its catalytic activity. Stable expression via a plasmid coding for JMJD6 mutant (H187A-D189A-H273A).

  • For Research Use Only

Target Details

  • Target: Catalytically inactive JMJD6
  • Target background: JMJD6 is an enzyme with intrinsic tyrosin kinase activity involved in the hydroxylation and de-methylation of histone and non-histone peptides with implications in the epigenetic changes of cancer. The jmjd6 gene also regulates its own splicing with the production of alternative splicied isoforms with different nuclear targets.

Application Details

  • Application: Western blot, Immunofluorescence

Handling

  • Growth medium: DMEM medium supplemented with 10% foetal calf serum, sodium pyruvate and antibiotics (penicillin/streptomycin)
  • Temperature: 37°C
  • Atmosphere: 5% CO2
  • Storage conditions: Liquid Nitrogen
  • Shipping conditions: Dry ice
  • Cultured in antibiotics?: Penicillin, Streptomycin
  • Biosafety level: BSL-1

Documentation

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References

  • •  Fages et al. 2020. PLoS Genet 16(6):e e1008511. PMID: 32598339.